A novel multiplex qPCR assay for detection of Plasmodium falciparum with histidine-rich protein 2 and 3 (pfhrp2 and pfhrp3) deletions in polyclonal infections
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DOI: 10.1016/j.ebiom.2020.102757
Abstract: Background Many health facilities in malaria endemic countries are dependent on Rapid diagnostic tests (RDTs) for diagnosis and some National Health Service (NHS) hospitals without expert microscopists rely on them for diagnosis out of hours.… read more here.
Keywords: protein; pfhrp2 pfhrp3; pfhrp3 deletions; qpcr assay ... See more keywords
Plasmodium falciparum histidine-rich protein (PfHRP2 and 3) diversity in Western and Coastal Kenya
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DOI: 10.1038/s41598-018-38175-1
Abstract: Plasmodium falciparum histidine-rich proteins 2 (PfHRP2) based RDTs are advocated in falciparum malaria-endemic regions, particularly when quality microscopy is not available. However, diversity and any deletion in the pfhrp2 and pfhrp3 genes can affect the… read more here.
Keywords: pfhrp3; amino acid; pfhrp2 pfhrp3; plasmodium falciparum ... See more keywords
Systematic review of the status of pfhrp2 and pfhrp3 gene deletion, approaches and methods used for its estimation and reporting in Plasmodium falciparum populations in Africa: review of published studies 2010–2019
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DOI: 10.1186/s12936-019-2987-4
Abstract: BackgroundMalaria rapid diagnostic tests based on histidine-rich protein-2 have played a vital role in improving malaria case management and surveillance particularly in Africa, where Plasmodium falciparum is predominant. However, their usefulness has been threatened by… read more here.
Keywords: pfhrp2 pfhrp3; deletion; gene deletion; review ... See more keywords
Molecular surveillance reveals the presence of pfhrp2 and pfhrp3 gene deletions in Plasmodium falciparum parasite populations in Uganda, 2017–2019
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DOI: 10.1186/s12936-020-03362-x
Abstract: Histidine-rich protein-2 (HRP2)-based rapid diagnostic tests (RDTs) are the only RDTs recommended for malaria diagnosis in Uganda. However, the emergence of Plasmodium falciparum histidine rich protein 2 and 3 (pfhrp2 and pfhrp3) gene deletions threatens… read more here.
Keywords: gene deletions; pfhrp2 pfhrp3; presence; gene ... See more keywords
Plasmodium falciparum pfhrp2 and pfhrp3 Gene Deletions and Relatedness to Other Global Isolates, Djibouti, 2019–2020
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DOI: 10.3201/eid2810.220695
Abstract: Deletions of pfhrp2 and paralogue pfhrp3 (pfhrp2/3) genes threaten Plasmodium falciparum diagnosis by rapid diagnostic test. We examined 1,002 samples from suspected malaria patients in Djibouti City, Djibouti, to investigate pfhrp2/3 deletions. We performed assays… read more here.
Keywords: pfhrp2; plasmodium falciparum; gene deletions; pfhrp2 pfhrp3 ... See more keywords