Histidine is essential for growth of Komagataella phaffii cultured in YPA medium
Sign Up to like & getrecommendations! Published in 2022 at "FEBS Open Bio"
DOI: 10.1002/2211-5463.13408
Abstract: Komagataella phaffii (a.k.a. Pichia pastoris) requires histidine for optimal growth when cultured in a medium containing yeast extract, peptone (YP), and acetate (YPA). We demonstrate that HIS4‐deficient, K. phaffii strain GS115 exhibits a growth defect… read more here.
Keywords: phaffii; komagataella phaffii; growth; cultured ypa ... See more keywords
Metabolic engineering of Komagataella phaffii for synergetic utilization of glucose and glycerol
Sign Up to like & getrecommendations! Published in 2022 at "Yeast"
DOI: 10.1002/yea.3793
Abstract: Komagataella phaffii GS115 is a proven heterologous expression system and has recently been exploited for the production of value‐added biochemicals from glucose through metabolic engineering. A major challenge for high‐level biochemical production is the appropriate… read more here.
Keywords: phaffii; komagataella phaffii; metabolic engineering; glucose glycerol ... See more keywords
Acetamidase as a dominant recyclable marker for Komagataella phaffii strain engineering
Sign Up to like & getrecommendations! Published in 2018 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-018-8824-9
Abstract: We have investigated the use of the gene coding for acetamidase (amdS) as a recyclable dominant marker for the methylotrophic yeast Komagataella phaffii in order to broaden its genetic toolbox. First, the endogenous constitutive AMD2… read more here.
Keywords: komagataella phaffii; marker; acetamidase; phaffii ... See more keywords
Development of wheat bran hydrolysate as Komagataella phaffii medium for heterologous protein production
Sign Up to like & getrecommendations! Published in 2021 at "Bioprocess and Biosystems Engineering"
DOI: 10.1007/s00449-021-02633-4
Abstract: Developing a Komagataella phaffii (K. phaffii, named as Pichia pastoris formerly) medium using wheat bran hydrolysate (WBH) is a potential approach for wheat bran utilization and heterologous protein by K. phaffii because K. phaffii is… read more here.
Keywords: medium; heterologous protein; phaffii; wheat bran ... See more keywords
PiggyBac transposon-mediated mutagenesis and application in yeast Komagataella phaffii
Sign Up to like & getrecommendations! Published in 2018 at "Biotechnology Letters"
DOI: 10.1007/s10529-018-2592-6
Abstract: ObjectiveAround one-fourth of the Komagataella phaffii genes encode hypothetical proteins with unknown functions. However, lack of powerful tools for genetic screening in K. phaffii significantly limits the functional analysis of these unknown genes. Transposon mutagenesis… read more here.
Keywords: mutagenesis; piggybac transposon; komagataella phaffii; phaffii ... See more keywords
Efficient Bioproduction of Human Milk Alpha-Lactalbumin in Komagataella phaffii.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of agricultural and food chemistry"
DOI: 10.1021/acs.jafc.1c07908
Abstract: Alpha-lactalbumin (α-LA; the most abundant whey protein in human milk) contributes to infant development, providing bioactive peptides and essential amino acids. Here, Komagataella phaffii (K. phaffii) was selected as the production host. We found that… read more here.
Keywords: phaffii; human milk; komagataella phaffii; alpha lactalbumin ... See more keywords
One-Step In Vivo Assembly of Multiple DNA Fragments and Genomic Integration in Komagataella phaffii.
Sign Up to like & getrecommendations! Published in 2022 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.1c00302
Abstract: The methylotrophic yeast species Komagataella phaffii (synonym: Pichia pastoris) is widely used as a host for recombinant protein production. Although several genetic engineering techniques are being employed on K. phaffii, advanced methods such as in… read more here.
Keywords: phaffii; dna; vivo; integration ... See more keywords
Multicopy plasmid integration in Komagataella phaffii mediated by a defective auxotrophic marker
Sign Up to like & getrecommendations! Published in 2017 at "Microbial Cell Factories"
DOI: 10.1186/s12934-017-0715-8
Abstract: BackgroundA commonly used approach to improve recombinant protein production is to increase the levels of expression by providing extra-copies of a heterologous gene. In Komagataella phaffii (Pichia pastoris) this is usually accomplished by transforming cells… read more here.
Keywords: protein; production; komagataella phaffii; defective auxotrophic ... See more keywords
Identification of genetic variants of the industrial yeast Komagataella phaffii (Pichia pastoris) that contribute to increased yields of secreted heterologous proteins
Sign Up to like & getrecommendations! Published in 2022 at "PLOS Biology"
DOI: 10.1371/journal.pbio.3001877
Abstract: The yeast Komagataella phaffii (formerly called Pichia pastoris) is used widely as a host for secretion of heterologous proteins, but only a few isolates of this species exist and all the commonly used expression systems… read more here.
Keywords: phaffii; komagataella phaffii; pichia pastoris; yeast komagataella ... See more keywords
From linoleic acid to hexanal and hexanol by whole cell catalysis with a lipoxygenase, hydroperoxide lyase and reductase cascade in Komagataella phaffii
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Molecular Biosciences"
DOI: 10.3389/fmolb.2022.965315
Abstract: Green leaf volatiles (GLVs) cover a group of mainly C6-and C9-aldehydes, -alcohols and -esters. Their name refers to their characteristic herbal and fruity scent, which is similar to that of freshly cut grass or vegetables.… read more here.
Keywords: hexanal hexanol; phaffii; komagataella phaffii; acid hexanal ... See more keywords
Constitutive Expression in Komagataella phaffii of Mature Rhizopus oryzae Lipase Jointly with Its Truncated Prosequence Improves Production and the Biocatalyst Operational Stability
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DOI: 10.3390/catal11101192
Abstract: Rhizopus oryzae lipase (ROL) containing 28 C-terminal amino acids of the prosequence fused to the N-terminal mature sequence in ROL (proROL) was successfully expressed in the methylotrophic yeast Komagataella phaffii (Pichia pastoris) under the constitutive… read more here.
Keywords: production; prorol; prosequence; rhizopus oryzae ... See more keywords