Reverse Phase Protein Arrays.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1064-0_9
Abstract: Reverse phase protein arrays (RPPA) are used to quantify proteins and protein posttranslational modifications in cellular lysates and body fluids. RPPA technology is suitable for biomarker discovery, protein pathway profiling, functional phenotype analysis, and drug… read more here.
Keywords: protein; protein arrays; antibody; reverse phase ... See more keywords
Reproducibility and Crossplatform Validation of Reverse-Phase Protein Array Data.
Sign Up to like & getrecommendations! Published in 2019 at "Advances in experimental medicine and biology"
DOI: 10.1007/978-981-32-9755-5_10
Abstract: Reverse-phase protein array (RPPA) technology is a high-throughput antibody- and microarray-based approach for the rapid profiling of levels of proteins and protein posttranslational modifications in biological specimens. The technology consumes small amounts of samples, can… read more here.
Keywords: protein; crossplatform validation; reverse phase; protein array ... See more keywords
Lysate Preparation for Reverse Phase Protein Arrays.
Sign Up to like & getrecommendations! Published in 2019 at "Advances in experimental medicine and biology"
DOI: 10.1007/978-981-32-9755-5_2
Abstract: Protein lysates from a variety of sample materials, e.g., cell lines, serum, frozen tissues, formalin-fixed and paraffin-embedded (FFPE) tissues, and fine needle aspirates, are compatible with the analysis using reverse phase protein arrays (RPPAs). Due… read more here.
Keywords: protein; phase; lysate preparation; protein arrays ... See more keywords
Combining the "Sibling Technologies" of Laser Capture Microdissection and Reverse Phase Protein Microarrays.
Sign Up to like & getrecommendations! Published in 2019 at "Advances in experimental medicine and biology"
DOI: 10.1007/978-981-32-9755-5_6
Abstract: Reverse phase protein microarrays (RPPA) and laser capture microdissection (LCM) are "sibling" technologies that originated from the same laboratory to overcome the challenge of quantifying low-abundance proteins in heterogeneous tissues. Combining both technologies provides both… read more here.
Keywords: laser capture; sibling technologies; protein microarrays; reverse phase ... See more keywords
The effects of sample handling on proteomics assessed by reverse phase protein arrays (RPPA): Functional proteomic profiling in leukemia.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of proteomics"
DOI: 10.1016/j.jprot.2020.104046
Abstract: Reverse phase protein arrays (RPPA) can assess protein expression and activation states in large numbers of samples (n > 1000) and evidence suggests feasibility in the setting of multi-institution clinical trials. Despite evidence in solid tumors, little… read more here.
Keywords: protein; phase; protein arrays; reverse phase ... See more keywords
The bovine acute phase protein α1-acid glycoprotein (AGP) can disrupt Staphylococcus aureus biofilm.
Sign Up to like & getrecommendations! Published in 2019 at "Veterinary microbiology"
DOI: 10.1016/j.vetmic.2019.06.007
Abstract: Staphylococcus aureus biofilm-related infections are of clinical concern due to the capability of bacterial colonies to adapt to a hostile environment. The present study investigated the capability of the acute phase protein alpha 1-acid glycoprotein… read more here.
Keywords: acute phase; staphylococcus aureus; acid glycoprotein; aureus biofilm ... See more keywords
Reverse phase protein arrays in acute leukemia: investigative and methodological challenges
Sign Up to like & getrecommendations! Published in 2021 at "Expert Review of Proteomics"
DOI: 10.1080/14789450.2021.2020655
Abstract: ABSTRACT Introduction Acute leukemia results from a series of mutational events that alter cell growth and proliferation. Mutations result in protein changes that orchestrate growth alterations characteristic of leukemia. Proteomics is a methodology appropriate for… read more here.
Keywords: phase protein; reverse phase; leukemia; acute leukemia ... See more keywords
RPPA SPACE: an R package for normalization and quantitation of Reverse-Phase Protein Array data
Sign Up to like & getrecommendations! Published in 2022 at "Bioinformatics"
DOI: 10.1093/bioinformatics/btac665
Abstract: Abstract Summary Reverse-Phase Protein Array (RPPA) is a robust high-throughput, cost-effective platform for quantitatively measuring proteins in biological specimens. However, converting raw RPPA data into normalized, analysis-ready data remains a challenging task. Here, we present… read more here.
Keywords: rppa space; reverse phase; package; rppa ... See more keywords
Acute phase protein concentrations following serial procaine penicillin G injections in horses.
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DOI: 10.1111/evj.13886
Abstract: BACKGROUND Acute phase protein (APP) measurement is used to detect inflammation. Intramuscular (IM) injections could cause tissue injury and induce an acute phase response. OBJECTIVES To evaluate the effects of IM procaine penicillin G (PPG)… read more here.
Keywords: acute phase; procaine penicillin; baseline mean; phase ... See more keywords
Ruminal pH, short-chain fatty acid concentrations, and serum acute phase protein concentrations during finishing for steers with and without rumen and liver pathology
Sign Up to like & getrecommendations! Published in 2017 at "Canadian Journal of Animal Science"
DOI: 10.1139/cjas-2016-0212
Abstract: Abstract: This study was carried out to determine whether ruminal fermentation and serum acute phase protein concentrations differed between steers with and without pathology at slaughter. Ruminally cannulated steers (n = 28) were housed with… read more here.
Keywords: acute phase; phase protein; serum acute; pathology ... See more keywords
Fetuin-A: A negative acute-phase protein linked to adipose tissue function in periparturient dairy cows.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of dairy science"
DOI: 10.3168/jds.2017-13644
Abstract: Fetuin-A (FetA) is a free fatty acid transporter and an acute-phase protein that enhances cellular lipid uptake and lipogenesis. In nonruminants, FetA is involved in lipid-induced inflammation. Despite FetA importance in lipid metabolism and inflammation,… read more here.
Keywords: feta; acute phase; protein; phase protein ... See more keywords