Phasor Analysis of Fluorescence Lifetime Enables Quantitative Multiplexed Molecular Imaging of Three Probes.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c02149
Abstract: The excited-state lifetime is an intrinsic property of fluorescent molecules that can be leveraged for multiplexed imaging. An advantage of fluorescence lifetime-based multiplexing is that signals from multiple probes can be gathered simultaneously, whereas traditional… read more here.
Keywords: three probes; fluorescence lifetime; phasor analysis; lifetime ... See more keywords
Label-Free Cytometric Evaluation of Mitosis via Stimulated Raman Scattering Microscopy and Spectral Phasor Analysis
Sign Up to like & getrecommendations! Published in 2023 at "Analytical Chemistry"
DOI: 10.1021/acs.analchem.3c00212
Abstract: Hyperspectral stimulated Raman scattering (SRS) microscopy is a robust imaging tool for the analysis of biological systems. Here, we present a unique perspective, a label-free spatiotemporal map of mitosis, by integrating hyperspectral SRS microscopy with… read more here.
Keywords: label free; mitosis; spectral phasor; microscopy ... See more keywords
Preprint Highlight: Multiplexed bioluminescence microscopy via phasor analysis.
Sign Up to like & getrecommendations! Published in 2022 at "Molecular biology of the cell"
DOI: 10.1091/mbc.p22-04-1006
Abstract: Luciferase‐based bioluminescence imaging requires no excitation light and thus can be advantageous over fluorescence imaging, but multicolor imaging has been a challenge due to the difficulties of spectral and substrate unmixing. To achieve effective multiplexing… read more here.
Keywords: bioluminescence; cell; microscopy; phasor analysis ... See more keywords
Use of nanosecond excitation pulses in fluorescence lifetime measurement via phasor analysis.
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DOI: 10.1364/oe.450761
Abstract: We investigated the possibility of using long excitation pulses in fluorescence lifetime imaging microscopy (FLIM) using phasor analysis. It has long been believed that the pulse width of an excitation laser must be shorter than… read more here.
Keywords: measurement; excitation; excitation pulses; phasor analysis ... See more keywords