Detection of phenol contamination in RNA samples and its impact on qRT-PCR results.
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DOI: 10.1016/j.ab.2019.02.002
Abstract: Residual phenol, carried over from RNA purification, can alter RNA concentration measurements and is assumed to inhibit PCR. Here, we demonstrate that Impurities A260 values of spectral content profiling (SCP) UV/Vis measurements correlated with phenol… read more here.
Keywords: rna; phenol contamination; qrt pcr; concentration ... See more keywords