Efficient strategies to enhance plasmid stability for fermentation of recombinant Escherichia coli harboring tyrosine phenol lyase.
Sign Up to like & getrecommendations! Published in 2021 at "Biotechnology letters"
DOI: 10.1007/s10529-021-03082-5
Abstract: OBJECTIVE To solve the bottleneck of plasmid instability during microbial fermentation of L-DOPA with recombinant Escherichia coli expressing heterologous tyrosine phenol lyase. RESULTS The tyrosine phenol lyase from Fusobacterium nucleatum was constitutively expressed in E.… read more here.
Keywords: plasmid stability; phenol lyase; fermentation; tyrosine phenol ... See more keywords
High-throughput assay of tyrosine phenol-lyase activity using a cascade of enzymatic reactions.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2022.114547
Abstract: Tyrosine phenol-lyase (TPL) exhibits great potential in industrial biosynthesis of l-tyrosine and its derivates. To uncover and screen TPLs with excellent catalytic properties, there is unmet demand for development of facile and reliable screening system… read more here.
Keywords: phenol lyase; catechol; tyrosine phenol; activity ... See more keywords
Statistical medium optimization and DO-STAT fed-batch fermentation for enhanced production of tyrosine phenol lyase in recombinant Escherichia coli
Sign Up to like & getrecommendations! Published in 2019 at "Preparative Biochemistry and Biotechnology"
DOI: 10.1080/10826068.2018.1541808
Abstract: Abstract Tyrosine phenol lyase (TPL) is a robust biocatalyst for the production of L-dihydroxyphenylalanine (L-DOPA). The improvement of TPL production is conducive to the industrial potential. In this study, the optimization of culture medium of… read more here.
Keywords: recombinant escherichia; production; escherichia coli; tyrosine phenol ... See more keywords