Strand Displacement and Unwinding Assays to Study the Concerted Action of the DNA Polymerase and SSB During Phi29 TP-DNA Replication.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1290-3_22
Abstract: The Bacillus subtilis phage Phi29 has a linear double-stranded DNA with a terminal protein (TP) covalently linked to each 5' end (TP-DNA). Phi29 single-stranded DNA-binding protein (SSB) is encoded by the viral gene 5 and… read more here.
Keywords: phi29 dna; phi29; dna; ssb ... See more keywords
Chimeric Phi29 DNA polymerase with helix–hairpin–helix motifs shows enhanced salt tolerance and replication performance
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DOI: 10.1111/1751-7915.13830
Abstract: Phi29 DNA polymerase (Phi29 Pol) has been successfully applied in DNA nanoball‐based sequencing, real‐time DNA sequencing from single polymerase molecules and nanopore sequencing employing the sequencing by synthesis (SBS) method. Among these, polymerase‐assisted nanopore sequencing… read more here.
Keywords: phi29; dna; phi29 pol; polymerase ... See more keywords
Phi29 assembly intermediates reveal how scaffold interactions with capsid protein drive capsid construction and maturation
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DOI: 10.1126/sciadv.adk8779
Abstract: The self-assembly of bacteriophage capsids from major capsid proteins (MCPs) and scaffolding proteins (SPs) and the subsequent expansion of these capsids are essential steps in bacteriophage life cycles. However, the mechanism by which assembly occurs… read more here.
Keywords: maturation; capsid; intermediates reveal; assembly intermediates ... See more keywords