Articles with "phi29" as a keyword



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Strand Displacement and Unwinding Assays to Study the Concerted Action of the DNA Polymerase and SSB During Phi29 TP-DNA Replication.

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Published in 2021 at "Methods in molecular biology"

DOI: 10.1007/978-1-0716-1290-3_22

Abstract: The Bacillus subtilis phage Phi29 has a linear double-stranded DNA with a terminal protein (TP) covalently linked to each 5' end (TP-DNA). Phi29 single-stranded DNA-binding protein (SSB) is encoded by the viral gene 5 and… read more here.

Keywords: phi29 dna; phi29; dna; ssb ... See more keywords
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Chimeric Phi29 DNA polymerase with helix–hairpin–helix motifs shows enhanced salt tolerance and replication performance

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Published in 2021 at "Microbial Biotechnology"

DOI: 10.1111/1751-7915.13830

Abstract: Phi29 DNA polymerase (Phi29 Pol) has been successfully applied in DNA nanoball‐based sequencing, real‐time DNA sequencing from single polymerase molecules and nanopore sequencing employing the sequencing by synthesis (SBS) method. Among these, polymerase‐assisted nanopore sequencing… read more here.

Keywords: phi29; dna; phi29 pol; polymerase ... See more keywords