Strand Displacement and Unwinding Assays to Study the Concerted Action of the DNA Polymerase and SSB During Phi29 TP-DNA Replication.
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DOI: 10.1007/978-1-0716-1290-3_22
Abstract: The Bacillus subtilis phage Phi29 has a linear double-stranded DNA with a terminal protein (TP) covalently linked to each 5' end (TP-DNA). Phi29 single-stranded DNA-binding protein (SSB) is encoded by the viral gene 5 and… read more here.
Keywords: phi29 dna; phi29; dna; ssb ... See more keywords
The Loop of the TPR1 Subdomain of Phi29 DNA Polymerase Plays a Pivotal Role in Primer-Terminus Stabilization at the Polymerization Active Site
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DOI: 10.3390/biom9110648
Abstract: Bacteriophage Phi29 DNA polymerase belongs to the protein-primed subgroup of family B DNA polymerases that use a terminal protein (TP) as a primer to initiate genome replication. The resolution of the crystallographic structure showed that… read more here.
Keywords: phi29 dna; polymerization; dna; dna polymerase ... See more keywords