Defining Membrane Protein Topology Using pho-lac Reporter Fusions.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7033-9_10
Abstract: Experimental determination of membrane protein topology can be achieved using various techniques. Here we present the pho-lac dual reporter system, a simple, convenient, and reliable tool to analyze the topology of membrane proteins in vivo.… read more here.
Keywords: reporter; pho lac; phoa; topology ... See more keywords
Functional differentiation and complementation of alkaline phosphatases and choreography of DOP scavenging in a marine diatom
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DOI: 10.1111/mec.16475
Abstract: Facing phosphate deficiency, phytoplankton use alkaline phosphatase (AP) to scavenge dissolved organophosphate (DOP). AP is a multitype (e.g., PhoA, PhoD) family of hydrolases and is known as a promiscuous enzyme with broad DOP substrate compatibility.… read more here.
Keywords: phoa; differentiation complementation; dop; complementation alkaline ... See more keywords