Determining Protein Phosphorylation Status Using Antibody Arrays and Phos-Tag Biotin.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1064-0_18
Abstract: We describe here a standard protocol for determining the phosphorylation status of protein multiplexes using antibody arrays and a biotinylated Phos-tag with a dodeca(ethylene glycol) spacer (Phos-tag Biotin). The procedure is based on an antibody… read more here.
Keywords: phos tag; phosphorylation status; using antibody;
Determining the Phosphorylation Status of Hippo Components YAP and TAZ Using Phos-tag.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8910-2_21
Abstract: Protein phosphorylation is one of the most important posttranslational modifications in cell signaling and regulation. Protein phosphorylation can be detected by site-specific phospho-antibodies, but generating these antibodies can be costly, time-consuming, and difficult. Recently, Phos-tag… read more here.
Keywords: phosphorylation status; phos tag; yap taz; phosphorylation ... See more keywords
Phosphorylation analysis of the Hippo-YAP pathway using Phos-tag.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of proteomics"
DOI: 10.1016/j.jprot.2022.104582
Abstract: Phosphorylation is an essential regulatory mechanism in cells that modifies diverse substrates, such as proteins, carbohydrates, lipids, and nucleotides. Protein phosphorylation regulates function, subcellular localization, and protein-protein interactions. Protein kinases and phosphatases catalyze this reversible… read more here.
Keywords: hippo; protein phosphorylation; phosphorylation; phos tag ... See more keywords
Recent developments in Phos-tag electrophoresis for the analysis of phosphoproteins in proteomics
Sign Up to like & getrecommendations! Published in 2022 at "Expert Review of Proteomics"
DOI: 10.1080/14789450.2022.2052850
Abstract: ABSTRACT Introduction Phosphate-binding tag (Phos-tag) sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) is an important development capable of analyzing the phosphorylation state of proteins. Conventionally, proteins were separated via SDS-PAGE and Phos-tag SDS-PAGE that use different… read more here.
Keywords: phos tag; sds page; electrophoresis;
Abstract 518: Phosphorylation of MK5 at Threonine-182 in the Activation Loop is Mediated by P38α/β in Cardiac Fibroblasts
Sign Up to like & getrecommendations! Published in 2020 at "Circulation Research"
DOI: 10.1161/res.127.suppl_1.518
Abstract: MAP kinase-activated protein kinase-5 (MK5) is a protein serine/threonine kinase involved in fibroblast function. MK5 is activated by phosphorylation at threonine-182 (Thr182): p38α/β, ERK3, and ERK4 have been implicated. We examined the phosphorylation of MK5… read more here.
Keywords: phosphorylation mk5; threonine 182; immunoreactivity; phos tag ... See more keywords