Analysis of 3'-Phosphoadenosine 5'-Phosphosulfate Transporters: Transporter Activity Assay, Real-Time Reverse Transcription Polymerase Chain Reaction, and Immunohistochemistry.
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DOI: 10.1007/978-1-0716-1398-6_51
Abstract: 3'-Phosphoadenosine 5'-phosphosulfate transporters (PAPSTs) play an important role in transporting 3'-phosphoadenosine 5'-phosphosulfate (PAPS), the universal sulfuryl donor for sulfation, from the cytosol into the lumen of the Golgi apparatus. Here, we describe three methods for… read more here.
Keywords: real time; phosphosulfate; activity assay; phosphoadenosine phosphosulfate ... See more keywords
Expression of enzymes for 3′-phosphoadenosine-5′-phosphosulfate (PAPS) biosynthesis and their preparation for PAPS synthesis and regeneration
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DOI: 10.1007/s00253-020-10709-6
Abstract: The synthesis of sulfated polysaccharides involves the sulfation of simpler polysaccharide substrates, through the action sulfotransferases using the cofactor, 3′-phosphoadenosine-5′-phosphosulfate (PAPS). Three enzymes are essential for the in vitro synthesis of PAPS, namely, pyrophosphatase (PPA),… read more here.
Keywords: paps synthesis; regeneration; expression; phosphoadenosine phosphosulfate ... See more keywords
Fluorinated Phosphoadenosine 5′-Phosphosulfate Analogues for Continuous Sulfotransferase Activity Monitoring and Inhibitor Screening by 19F NMR Spectroscopy
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DOI: 10.1021/acschembio.1c00978
Abstract: Sulfotransferases (STs) are ubiquitous enzymes that participate in a vast number of biological processes involving sulfuryl group (SO3) transfer. 3′-phosphoadenosine 5′-phosphosulfate (PAPS) is the universal ST cofactor, serving as the “active sulfate” source in cells.… read more here.
Keywords: spectroscopy; 19f nmr; activity; phosphoadenosine phosphosulfate ... See more keywords