Phosphopeptide Enrichment from Bacterial Samples Utilizing Titanium Oxide Affinity Chromatography.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8695-8_16
Abstract: Mass spectrometry (MS)-based proteomics detected hundreds of phosphorylation sites on serine, threonine and tyrosine in numerous bacterial proteins, firmly establishing the presence and importance of this posttranslational modification in prokaryotes. Recent biological follow up of… read more here.
Keywords: phosphopeptide enrichment; chromatography; enrichment bacterial; bacterial samples ... See more keywords
Phosphopeptide enrichment for phosphoproteomic analysis - A tutorial and review of novel materials.
Sign Up to like & getrecommendations! Published in 2020 at "Analytica chimica acta"
DOI: 10.1016/j.aca.2020.04.053
Abstract: Significant technical advancements in phosphopeptide enrichment have enabled the identification of thousands of p-peptides (mono and multiply phosphorylated) in a single experiment. However, it is still not possible to enrich all p-peptide species in a… read more here.
Keywords: phosphopeptide enrichment; analysis; novel materials; review ... See more keywords
Light, pH, and Temperature Triple-Responsive Magnetic Composites for Highly Efficient Phosphopeptide Enrichment.
Sign Up to like & getrecommendations! Published in 2023 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.3c01330
Abstract: Smart materials can dynamically and reversibly change their structures and functions in response to external stimuli. In this study, we designed a smart magnetic composite (MNP-pSPA-b-pNIPAm) with a triple response to ultraviolet (UV) light, pH,… read more here.
Keywords: phosphopeptide enrichment; light temperature; temperature; pspa pnipam ... See more keywords
Selective TiO2 Phosphopeptide Enrichment of Complex Samples in the Nanogram Range
Sign Up to like & getrecommendations! Published in 2020 at "Separations"
DOI: 10.3390/separations7040074
Abstract: Phosphopeptide enrichment is a commonly used sample preparation step for investigating phosphorylation. TiO2-based enrichment has been demonstrated to have excellent performance both for large amounts of complex and for small amounts of simple samples. However,… read more here.
Keywords: phosphopeptide enrichment; samples nanogram; complex samples; nanogram range ... See more keywords