Identification of Protein Phosphorylation Sites by Advanced LC-ESI-MS/MS Methods.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9055-9_11
Abstract: Phosphorylation, the process by which a phosphate group is attached to a preexisting protein, is an evolutionarily and metabolically cheap way to change the protein's surface and properties. It is presumably for that reason that… read more here.
Keywords: protein; identification protein; phosphorylation sites; protein phosphorylation ... See more keywords
Mutations in the phosphorylation sites of SARS-CoV-2 encoded nucleocapsid protein and structure model of sequestration by protein 14-3-3
Sign Up to like & getrecommendations! Published in 2020 at "Biochemical and Biophysical Research Communications"
DOI: 10.1016/j.bbrc.2020.08.024
Abstract: Abstract SARS-CoV-2 is the etiologic agent of COVID-19. There is currently no effective means of preventing infections by SARS-CoV-2, except through restriction of population movement and contact. An understanding of the origin, evolution and biochemistry… read more here.
Keywords: protein; sequestration; nucleocapsid protein; phosphorylation sites ... See more keywords
Targeting the Allosteric Pathway That Interconnects the Core-Functional Scaffold and the Distal Phosphorylation Sites for Specific Dephosphorylation of Bcl-2.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of medicinal chemistry"
DOI: 10.1021/acs.jmedchem.0c01290
Abstract: Protein phosphorylation is the most significant post-translational modification for regulating cellular activities, but site-specific modulation of phosphorylation is still challenging. Using three-dimensional NMR spectra, molecular dynamics simulations, and alanine mutations, we identified that the interaction… read more here.
Keywords: dephosphorylation bcl; phosphorylation sites; dephosphorylation; targeting allosteric ... See more keywords
Kinase inhibition profiles as a tool to identify kinases for specific phosphorylation sites
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DOI: 10.1038/s41467-020-15428-0
Abstract: There are thousands of known cellular phosphorylation sites, but the paucity of ways to identify kinases for particular phosphorylation events remains a major roadblock for understanding kinase signaling. To address this, we here develop a… read more here.
Keywords: kinase; inhibition; phosphorylation sites; specific phosphorylation ... See more keywords
PKSPS: a novel method for predicting kinase of specific phosphorylation sites based on maximum weighted bipartite matching algorithm and phosphorylation sequence enrichment analysis.
Sign Up to like & getrecommendations! Published in 2021 at "Briefings in bioinformatics"
DOI: 10.1093/bib/bbab436
Abstract: With the development of biotechnology, a large number of phosphorylation sites have been experimentally confirmed and collected, but only a few of them have kinase annotations. Since experimental methods to detect kinases at specific phosphorylation… read more here.
Keywords: kinase; phosphorylation sites; specific phosphorylation; pksps ... See more keywords
Predicting protein phosphorylation sites in soybean using interpretable deep tabular learning network
Sign Up to like & getrecommendations! Published in 2022 at "Briefings in bioinformatics"
DOI: 10.1093/bib/bbac015
Abstract: Phosphorylation of proteins is one of the most significant post-translational modifications (PTMs) and plays a crucial role in plant functionality due to its impact on signaling, gene expression, enzyme kinetics, protein stability and interactions. Accurate… read more here.
Keywords: deep tabular; phosphorylation sites; tabular learning; sites soybean ... See more keywords
Holistic similarity-based prediction of phosphorylation sites for understudied kinases
Sign Up to like & getrecommendations! Published in 2023 at "Briefings in bioinformatics"
DOI: 10.1093/bib/bbac624
Abstract: Phosphorylation is an essential mechanism for regulating protein activities. Determining kinase-specific phosphorylation sites by experiments involves time-consuming and expensive analyzes. Although several studies proposed computational methods to model kinase-specific phosphorylation sites, they typically required abundant… read more here.
Keywords: understudied kinases; experimentally verified; similarity; phosphorylation sites ... See more keywords
Ablating putative Ku70 phosphorylation sites results in defective DNA damage repair and spontaneous induction of hepatocellular carcinoma
Sign Up to like & getrecommendations! Published in 2021 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkab743
Abstract: Abstract Multiple pathways mediate the repair of DNA double-strand breaks (DSBs), with numerous mechanisms responsible for driving choice between the pathways. Previously, we reported that mutating five putative phosphorylation sites on the non-homologous end joining… read more here.
Keywords: hepatocellular carcinoma; repair spontaneous; ku70; phosphorylation sites ... See more keywords
Identification of Phosphorylation Sites Altering Pollen Soluble Inorganic Pyrophosphatase Activity1[CC-BY]
Sign Up to like & getrecommendations! Published in 2017 at "Plant Physiology"
DOI: 10.1104/pp.16.01450
Abstract: Phosphoregulation of key sites on pollen soluble inorganic pyrophosphatases inhibits their catalytic responsiveness in concert with key intracellular events. Protein phosphorylation regulates numerous cellular processes. Identifying the substrates and protein kinases involved is vital to… read more here.
Keywords: phosphorylation sites; pollen soluble; inorganic phosphate; identification phosphorylation ... See more keywords
Abstract 2137: A high proportion of protein phosphorylation sites identified in CPTAC breast cancer and lung adenocarcinoma may result from direct phosphorylation by CDK1
Sign Up to like & getrecommendations! Published in 2020 at "Cancer Research"
DOI: 10.1158/1538-7445.am2020-2137
Abstract: Cyclin-dependent kinase 1 (CDK1) is a serine/threonine kinase that drives the G2/M phase of the cell cycle, and increased CDK1 kinase activity is a frequent feature of several tumor types. In the current study, we… read more here.
Keywords: lung adenocarcinoma; cdk1; phosphorylation sites; cancer ... See more keywords
Systematic identification of phosphorylation-mediated protein interaction switches
Sign Up to like & getrecommendations! Published in 2017 at "PLoS Computational Biology"
DOI: 10.1371/journal.pcbi.1005462
Abstract: Proteomics techniques can identify thousands of phosphorylation sites in a single experiment, the majority of which are new and lack precise information about function or molecular mechanism. Here we present a fast method to predict… read more here.
Keywords: phosphorylation mediated; systematic identification; phosphorylation sites; identification phosphorylation ... See more keywords