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Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1064-0_18
Abstract: We describe here a standard protocol for determining the phosphorylation status of protein multiplexes using antibody arrays and a biotinylated Phos-tag with a dodeca(ethylene glycol) spacer (Phos-tag Biotin). The procedure is based on an antibody…
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Keywords:
phos tag;
phosphorylation status;
using antibody;
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Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8910-2_21
Abstract: Protein phosphorylation is one of the most important posttranslational modifications in cell signaling and regulation. Protein phosphorylation can be detected by site-specific phospho-antibodies, but generating these antibodies can be costly, time-consuming, and difficult. Recently, Phos-tag…
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Keywords:
phosphorylation status;
phos tag;
yap taz;
phosphorylation ... See more keywords
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Published in 2020 at "ACS applied materials & interfaces"
DOI: 10.1021/acsami.9b21333
Abstract: The protein phosphorylation status of exosomes can regulate the activity and function of proteins related to cancer development, it's highly possible to diagnose cancers through analyzing the protein phosphorylation status. However, monitoring the protein phosphorylation…
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Keywords:
phosphorylation status;
exosomes plasma;
protein phosphorylation;
inspired macroporous ... See more keywords
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Published in 2017 at "Bioscience Reports"
DOI: 10.1042/bsr20170069
Abstract: Light and brassinosteroid (BR) are master environmental stimulus and endogenous cue for plant growth and development respectively. Great progress has been made in elucidating the molecular mechanisms on the cross-talk between light and BR. However,…
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Keywords:
plant growth;
phosphorylation status;
stability;
phosphorylation ... See more keywords
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Published in 2020 at "PLOS ONE"
DOI: 10.1371/journal.pone.0232635.s005
Abstract: Fibroblasts, hTERT-BJ1 cell- were mock infected (m) or infected with the following HCMV strains at a multiplicity of infection (MOI) of 5, harvested at 3 day post-infection, and their lysates were analyzed by immunoblotting with…
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Keywords:
hcmv encoding;
type ul42;
wild type;
phosphorylation status ... See more keywords