Determining Protein Phosphorylation Status Using Antibody Arrays and Phos-Tag Biotin.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1064-0_18
Abstract: We describe here a standard protocol for determining the phosphorylation status of protein multiplexes using antibody arrays and a biotinylated Phos-tag with a dodeca(ethylene glycol) spacer (Phos-tag Biotin). The procedure is based on an antibody… read more here.
Keywords: phos tag; phosphorylation status; using antibody;
Determining the Phosphorylation Status of Hippo Components YAP and TAZ Using Phos-tag.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8910-2_21
Abstract: Protein phosphorylation is one of the most important posttranslational modifications in cell signaling and regulation. Protein phosphorylation can be detected by site-specific phospho-antibodies, but generating these antibodies can be costly, time-consuming, and difficult. Recently, Phos-tag… read more here.
Keywords: phosphorylation status; phos tag; yap taz; phosphorylation ... See more keywords
Beehive-inspired macroporous SERS probe for cancer detection through capturing and analyzing exosomes in plasma.
Sign Up to like & getrecommendations! Published in 2020 at "ACS applied materials & interfaces"
DOI: 10.1021/acsami.9b21333
Abstract: The protein phosphorylation status of exosomes can regulate the activity and function of proteins related to cancer development, it's highly possible to diagnose cancers through analyzing the protein phosphorylation status. However, monitoring the protein phosphorylation… read more here.
Keywords: phosphorylation status; exosomes plasma; protein phosphorylation; inspired macroporous ... See more keywords
Light involved regulation of BZR1 stability and phosphorylation status to coordinate plant growth in Arabidopsis
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DOI: 10.1042/bsr20170069
Abstract: Light and brassinosteroid (BR) are master environmental stimulus and endogenous cue for plant growth and development respectively. Great progress has been made in elucidating the molecular mechanisms on the cross-talk between light and BR. However,… read more here.
Keywords: plant growth; phosphorylation status; stability; phosphorylation ... See more keywords
The phosphorylation status of c-Jun in fibroblasts infected with HCMV wild-type or UL42 mutants.
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DOI: 10.1371/journal.pone.0232635.s005
Abstract: Fibroblasts, hTERT-BJ1 cell- were mock infected (m) or infected with the following HCMV strains at a multiplicity of infection (MOI) of 5, harvested at 3 day post-infection, and their lysates were analyzed by immunoblotting with… read more here.
Keywords: hcmv encoding; type ul42; wild type; phosphorylation status ... See more keywords