A Tyrosine Phosphoproteome Analysis Approach Enabled by Selective Dephosphorylation with Protein Tyrosine Phosphatase.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.1c03704
Abstract: Protein tyrosine phosphorylation (pTyr) plays a prominent role in signal transduction and regulation in all eukaryotic cells. In conventional immunoaffinity purification (IP) methods, phosphotyrosine peptides are isolated from the digest of cellular protein extracts with… read more here.
Keywords: protein tyrosine; tyrosine; dephosphorylation; phosphotyrosine peptides ... See more keywords
Automated Enrichment of Phosphotyrosine Peptides for High-Throughput Proteomics.
Sign Up to like & getrecommendations! Published in 2023 at "Journal of proteome research"
DOI: 10.1021/acs.jproteome.2c00850
Abstract: Phosphotyrosine (pY) enrichment is critical for expanding the fundamental and clinical understanding of cellular signaling by mass spectrometry-based proteomics. However, current pY enrichment methods exhibit a high cost per sample and limited reproducibility due to… read more here.
Keywords: enrichment phosphotyrosine; phosphotyrosine peptides; high throughput; automated enrichment ... See more keywords