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   Articles with "photoaffinity labeling" as a keyword



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Activity-Dependent Photoaffinity Labeling of Metalloproteases.

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recommendations! Published in 2017 at "Methods in molecular biology"

DOI: 10.1007/978-1-4939-6439-0_8

Abstract: Metalloproteases, notably members of the matrix metalloprotease (MMP) and A Disintegrin And Metalloprotease (ADAM) families play crucial roles in tissue remodeling, the liberation of growth factors and cytokines from cell membranes (shedding) and cell-cell or… read more here.

Keywords: dependent photoaffinity; cell; activity; photoaffinity labeling ... See more keywords
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Sites Contributing to TRPA1 Activation by the Anesthetic Propofol Identified by Photoaffinity Labeling.

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recommendations! Published in 2017 at "Biophysical journal"

DOI: 10.1016/j.bpj.2017.08.040

Abstract: In addition to inducing anesthesia, propofol activates a key component of the pain pathway, the transient receptor potential ankyrin 1 ion channel (TRPA1). Recent mutagenesis studies suggested a potential activation site within the transmembrane domain,… read more here.

Keywords: propofol; photoaffinity; sites contributing; binding sites ... See more keywords

Discovery of AHCY as an Off-Target of Doxorubicin by Integrative Analysis of Photoaffinity Labeling Chemoproteomics and Untargeted Metabolomics.

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recommendations! Published in 2022 at "Analytical chemistry"

DOI: 10.1021/acs.analchem.2c03377

Abstract: Target identification is critically important for understanding the mechanism of action of drugs. Here, we reported a new strategy for deconvolution of drug targets (or off-targets) with photoaffinity labeling chemoproteomics in combination with untargeted metabolomics… read more here.

Keywords: untargeted metabolomics; metabolism; photoaffinity labeling; analysis ... See more keywords
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Probing the Mechanism of Photoaffinity Labeling by Dialkyldiazirines through Bioorthogonal Capture of Diazoalkanes.

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recommendations! Published in 2020 at "Organic letters"

DOI: 10.1021/acs.orglett.0c02714

Abstract: Dialkyldiazirines have emerged as reagents of choice for biological photoaffinity labeling studies. The mechanism of crosslinking has dramatic consequences for biological applications where instantaneous labeling is desirable, as carbene insertions display different chemoselectivity and are… read more here.

Keywords: mechanism photoaffinity; probing mechanism; photoaffinity; diazo ... See more keywords
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Photoaffinity Labeling of the Human A2A Adenosine Receptor and Cross-link Position Analysis by Mass Spectrometry.

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recommendations! Published in 2017 at "ACS medicinal chemistry letters"

DOI: 10.1021/acsmedchemlett.7b00138

Abstract: Photoaffinity labeling (PAL) is widely used for the identification of ligand-binding proteins and elucidation of ligand-binding sites. PAL has also been employed for the characterization of G protein-coupled receptors (GPCRs); however, a limited number of… read more here.

Keywords: a2a adenosine; cross; cross link; link position ... See more keywords
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Click-Addressable Cassette for Photoaffinity Labeling.

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recommendations! Published in 2018 at "ACS medicinal chemistry letters"

DOI: 10.1021/acsmedchemlett.7b00516

Abstract: A small molecule 1 was designed to contain an alkyne, a trifluoromethyl phenyldiazirine, and a free piperidine-NH for facile conjugation to protein binding ligands. This "cassette" 1 was synthesized via a relatively direct route involving… read more here.

Keywords: addressable cassette; chemistry; photoaffinity; cassette photoaffinity ... See more keywords

Developments of bioorthogonal handle-containing photo-crosslinkers for photoaffinity labeling.

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recommendations! Published in 2017 at "MedChemComm"

DOI: 10.1039/c7md00217c

Abstract: Photoaffinity labeling (PAL) has been widely applied in various research areas such as medicinal chemistry, chemical biology and structural biology, owing to its capability of investigating non-covalent ligand-protein interactions under native environments and elucidating protein… read more here.

Keywords: bioorthogonal handle; photo; photo crosslinkers; biology ... See more keywords
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Functional sites for anesthetics in GABAA receptors

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recommendations! Published in 2017 at "Oncotarget"

DOI: 10.18632/oncotarget.14616

Abstract: A key target for the intravenous anesthetics propofol and etomidate is the γ-aminobutyric acid type A (GABAA) receptor [1]. GABAA receptors are the most important inhibitory neurotransmitter receptors in the central nervous system. They are… read more here.

Keywords: subunit interfaces; gabaa receptors; anesthetics gabaa; potentiation ... See more keywords