Coregistered Spectral Optical Coherence Tomography and Two‐Photon Microscopy for Multimodal Near‐Instantaneous Deep‐Tissue Imaging
Sign Up to like & getrecommendations! Published in 2020 at "Cytometry Part A"
DOI: 10.1002/cyto.a.24012
Abstract: Two‐photon microscopy (2PM) has brought unique insight into the mechanisms underlying immune system dynamics and function since it enables monitoring of cellular motility and communication in complex systems within their genuine environment—the living organism. However,… read more here.
Keywords: optical coherence; microscopy; two photon; tissue ... See more keywords
Investigating T Cell Receptor Signals In Situ by Two-Photon Microscopy of Thymocytes Expressing Genetic Reporters in Low-Density Chimeras.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0266-9_18
Abstract: T cell development is a dynamic process accompanied by extensive thymocyte migration, cellular interactions, and T cell receptor (TCR) signaling. In particular, thymic selection processes that ensure a functional, self-tolerant repertoire require TCR interactions with… read more here.
Keywords: microscopy; two photon; tcr signaling; cell ... See more keywords
Tracking Cancer Cells Colonization in Rodent Bone Using Two-Photon Microscopy.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8997-3_33
Abstract: Two-photon microscopy has been widely accepted as a powerful tool to provide both qualitative and quantitative information in bone research. This chapter will describe a step-by-step protocol for using two-photon microscopy to track the colonization… read more here.
Keywords: microscopy; two photon; bone; using two ... See more keywords
Two-Photon Microscopy with a Double-Wavelength Metasurface Objective Lens.
Sign Up to like & getrecommendations! Published in 2018 at "Nano letters"
DOI: 10.1021/acs.nanolett.8b01737
Abstract: Two-photon microscopy is a key imaging technique in life sciences due to its superior deep-tissue imaging capabilities. Light-weight and compact two-photon microscopes are of great interest because of their applications for in vivo deep brain… read more here.
Keywords: microscopy; two photon; double wavelength; metasurface ... See more keywords
Bright Near-Infrared π-Conjugated Oligomer Nanoparticles for Deep-Brain Three-Photon Microscopy Excited at the 1700 nm Window in Vivo.
Sign Up to like & getrecommendations! Published in 2022 at "ACS nano"
DOI: 10.1021/acsnano.2c03813
Abstract: The development of three-photon fluorophores with 1700 nm excitation is pressingly desirable for in vivo imaging of tissue resided deep inside the brain. Herein, we report a designed and synthesized fluorescent molecule (OFET) for in… read more here.
Keywords: three photon; vivo; photon microscopy; microscopy ... See more keywords
Functional imaging of visual cortical layers and subplate in awake mice with optimized three-photon microscopy
Sign Up to like & getrecommendations! Published in 2019 at "Nature Communications"
DOI: 10.1038/s41467-018-08179-6
Abstract: Two-photon microscopy is used to image neuronal activity, but has severe limitations for studying deeper cortical layers. Here, we developed a custom three-photon microscope optimized to image a vertical column of the cerebral cortex > 1 mm in… read more here.
Keywords: photon; awake mice; microscopy; cortical layers ... See more keywords
Two-photon microscopy as a visual tool for polymer compatibilization monitoring: the PE-EVOH case.
Sign Up to like & getrecommendations! Published in 2023 at "Soft matter"
DOI: 10.1039/d2sm01577c
Abstract: In this study, we present two-photon microscopy (2PM) as an original technique to investigate the compatibilization between PE-HEMA and EVOH at the sub-micrometer level, both on the surface and in the bulk. 2PM is a… read more here.
Keywords: two photon; polymer; photon microscopy; microscopy ... See more keywords
Automatic Graph-Based Modeling of Brain Microvessels Captured With Two-Photon Microscopy
Sign Up to like & getrecommendations! Published in 2019 at "IEEE Journal of Biomedical and Health Informatics"
DOI: 10.1109/jbhi.2018.2884678
Abstract: Graph models of cerebral vasculature derived from two-photon microscopy have shown to be relevant to study brain microphysiology. Automatic graphing of these microvessels remain problematic due to the vascular network complexity and two-photon sensitivity limitations… read more here.
Keywords: photon microscopy; microscopy; two photon; automatic graph ... See more keywords
Gastric cancer multicellular spheroid analysis by two-photon microscopy.
Sign Up to like & getrecommendations! Published in 2022 at "Biomedical optics express"
DOI: 10.1364/boe.450518
Abstract: Gastric cancer (GC) is highly deadly. Three-dimensional (3D) cancer cell cultures, known as spheroids, better mimic tumor microenvironment (TME) than standard 2D cultures. Cancer-associated fibroblasts (CAF), a major cellular component of TME, promote or restrain… read more here.
Keywords: two photon; photon microscopy; microscopy; gastric cancer ... See more keywords
Scattering correcting wavefront shaping for three-photon microscopy.
Sign Up to like & getrecommendations! Published in 2022 at "Optics letters"
DOI: 10.1364/ol.468834
Abstract: Three-photon (3P) microscopy is getting traction due to its superior performance in deep tissues. Yet, aberrations and light scattering still pose one of the main limitations in the attainable depth ranges for high-resolution imaging. Here,… read more here.
Keywords: wavefront shaping; three photon; photon microscopy; microscopy ... See more keywords
Changes in the microstructure of the human aortic medial layer under biaxial loading investigated by multi-photon microscopy.
Sign Up to like & getrecommendations! Published in 2022 at "Acta biomaterialia"
DOI: 10.2139/ssrn.4060853
Abstract: Understanding the correlation between tissue architecture, health status, and mechanical properties is essential for improving material models and developing tissue engineering scaffolds. Since structural-based material models are state of the art, there is an urgent… read more here.
Keywords: structural parameters; photon microscopy; microscopy; human aortic ... See more keywords