Rational Control of OffâState Heterogeneity in a Photoswitchable Fluorescent Protein Provides Switching Contrast Enhancement
Sign Up to like & getrecommendations! Published in 2022 at "Chemphyschem"
DOI: 10.1002/cphc.202200192
Abstract: Abstract Reversibly photoswitchable fluorescent proteins are essential markers for advanced biological imaging, and optimization of their photophysical properties underlies improved performance and novel applications. Here we establish a link between photoswitching contrast, one of the… read more here.
Keywords: switching contrast; rational control; control state; contrast ... See more keywords
Photoswitchable Fluorescent Crystals Obtained by the Photoreversible Coassembly of a Nucleobase and an Azobenzene Intercalator.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.9b02836
Abstract: Self-assembled nucleobases, such as G-quartets or quadruplexes, have numerous applications, but light-responsive structures are limited to small, noncrystalline motifs. In addition, the assembly of the widely exploited azobenzene photochromic compounds can produce fluorescent crystals of… read more here.
Keywords: azobenzene; crystals obtained; intercalator; photoswitchable fluorescent ... See more keywords
Optically controlled reversible protein hydrogels based on photoswitchable fluorescent protein Dronpa.
Sign Up to like & getrecommendations! Published in 2017 at "Chemical communications"
DOI: 10.1039/c7cc06991j
Abstract: Exploiting the optically controlled association and dissociation behavior of a photoswitchable fluorescent protein, Dronpa145N, here we demonstrate the engineering of an optically switchable reversible protein hydrogel using Dronpa145N-based protein building blocks. Our results open the… read more here.
Keywords: protein; fluorescent protein; protein hydrogels; optically controlled ... See more keywords
Dynamic contrast with reversibly photoswitchable fluorescent labels for imaging living cells
Sign Up to like & getrecommendations! Published in 2020 at "Chemical Science"
DOI: 10.1039/d0sc00182a
Abstract: Interrogating living cells requires sensitive imaging of a large number of components in real time. The state-of-the-art of multiplexed imaging is usually limited to a few components. This review reports on the promise and the… read more here.
Keywords: reversibly photoswitchable; photoswitchable fluorescent; dynamic contrast; living cells ... See more keywords
Photoswitchable Fluorescent Proteins: Mechanisms on Ultrafast Timescales
Sign Up to like & getrecommendations! Published in 2022 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms23126459
Abstract: The advancement of super-resolution imaging (SRI) relies on fluorescent proteins with novel photochromic properties. Using light, the reversibly switchable fluorescent proteins (RSFPs) can be converted between bright and dark states for many photocycles and their… read more here.
Keywords: fluorescent proteins; ultrafast timescales; rsfps; proteins mechanisms ... See more keywords