Production and characterization of a human lysosomal recombinant iduronate‐2‐sulfatase produced in Pichia pastoris
Sign Up to like & getrecommendations! Published in 2018 at "Biotechnology and Applied Biochemistry"
DOI: 10.1002/bab.1660
Abstract: Hunter syndrome (Mucopolysaccharidosis II, MPS II) is an X‐linked lysosomal storage disease produced by the deficiency of the lysosomal enzyme iduronate‐2‐sulfatase (IDS). Currently, MPS II patients are mainly treated with enzyme replacement therapy (ERT) using… read more here.
Keywords: production; enzyme; production characterization; pichia pastoris ... See more keywords
Development of a general defined medium for Pichia pastoris
Sign Up to like & getrecommendations! Published in 2018 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.26440
Abstract: Pichia pastoris is widely used as a host for recombinant protein production. More than 500 proteins have been expressed in the organism at a variety of cultivation scales, from small shake flasks to large bioreactors.… read more here.
Keywords: medium; defined medium; pichia pastoris; development ... See more keywords
Characterization and high‐yield production of non‐N‐glycosylated recombinant human BCMA‐Fc in Pichia pastoris
Sign Up to like & getrecommendations! Published in 2017 at "Engineering in Life Sciences"
DOI: 10.1002/elsc.201600039
Abstract: B‐cell maturation antigen (BCMA) fused at the C‐terminus to the Fc portion of human IgG1 (BCMA‐Fc) blocks B‐cell activating factor (BAFF) and proliferation‐inducing ligand (APRIL)‐mediated B‐cell activation, leading to immune disorders. The fusion protein has… read more here.
Keywords: recombinant human; production; bcma; human bcma ... See more keywords
Investigating the effect of carbon source on rabies virus glycoprotein production in Pichia pastoris by a transcriptomic approach
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DOI: 10.1002/mbo3.489
Abstract: Several factors affect protein expression in Pichia pastoris, one among them is the carbon source. In this work, we studied the effect of this factor on the expression level of rabies virus glycoprotein (RABV‐G) in… read more here.
Keywords: virus glycoprotein; rabies virus; carbon; carbon source ... See more keywords
Identification and characterization of novel promoters for recombinant protein production in yeast Pichia pastoris
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DOI: 10.1002/yea.3301
Abstract: Pichia pastoris expression system has been widely used in recombinant protein production. So far the majority of heterologous proteins are expressed by methanol inducible promoter PAOX1 and constitutive promoter PGAP. The use of other promoters… read more here.
Keywords: protein production; pichia pastoris; recombinant protein; promoter ... See more keywords
Efficient Development of a Mixed Feed Process for Pichia pastoris.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9024-5_15
Abstract: Pichia pastoris is one of the most important host organisms for the recombinant production of proteins in industrial biotechnology. A prominent promoter system for recombinant protein production in P. pastoris is the promoter of alcohol… read more here.
Keywords: pichia pastoris; mixed feed; efficient development;
CRISPR/Cas9-Mediated Homology-Directed Genome Editing in Pichia pastoris.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9024-5_9
Abstract: State-of-the-art strain engineering techniques for the methylotrophic yeast Pichia pastoris (syn. Komagataella spp.) include overexpression of endogenous and heterologous genes and deletion of host genes. For efficient gene deletion, methods such as the split-marker technique have… read more here.
Keywords: crispr cas9; cas9 mediated; biology; pichia pastoris ... See more keywords
Improving glutathione production by engineered Pichia pastoris: strain construction and optimal precursor feeding.
Sign Up to like & getrecommendations! Published in 2022 at "Applied microbiology and biotechnology"
DOI: 10.1007/s00253-022-11827-z
Abstract: Glutathione (GSH) is a metabolite that plays an important role in the fields of pharmacy, food, and cosmetics. Thus, it is necessary to increase its production to meet the demands. In this study, ScGSH1, ScGSH2,… read more here.
Keywords: pichia pastoris; precursor feeding; production; synthesis gsh ... See more keywords
Purification of Histidine-Tagged Membrane-Bound Catechol-O-Methyltransferase from Detergent-Solubilized Pichia pastoris Membranes
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DOI: 10.1007/s10337-017-3453-5
Abstract: Catechol-O-methyltransferase (COMT) is an enzyme involved in catecholamine catabolism that is key for the treatment of different neurologic disorders. Actually, there are still unmet needs concerning the development of more selective membrane-bound COMT (hMBCOMT) downstream… read more here.
Keywords: pastoris membranes; membrane bound; catechol methyltransferase; hmbcomt ... See more keywords
Crude glycerol from biodiesel as a carbon source for production of a recombinant highly thermostable β-mannanase by Pichia pastoris
Sign Up to like & getrecommendations! Published in 2017 at "Biotechnology Letters"
DOI: 10.1007/s10529-017-2451-x
Abstract: ObjectiveTo explore an efficient use of crude glycerol for the production of a highly thermostable β-mannanase (ReTMan26) by Pichia pastoris X33.ResultsCell growth was significantly inhibited by 4 and 6% (w/v) crude glycerol in 250 ml shake-flasks… read more here.
Keywords: production; crude glycerol; glycerol; pichia pastoris ... See more keywords
Expression of T9W in Pichia pastoris and the protective roles of T9W in ICR Mice
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DOI: 10.1007/s10529-019-02759-2
Abstract: BackgroundThe purpose of this paper is to express the novel α-helical peptide T9W more efficiently using the Pichia pastoris expression system and to examine the role of T9W in ICR mice.ResultsThe novel antimicrobial peptide T9W… read more here.
Keywords: icr mice; t9w; pichia pastoris; mice ... See more keywords