Applications of CRISPR/Cas9 technology for modification of the plant genome
Sign Up to like & getrecommendations! Published in 2022 at "Genetica"
DOI: 10.1007/s10709-021-00146-2
Abstract: The CRISPR/Cas (Clustered regularly interspaced short palindromic repeats/ CRISPR associated protein 9) system was discovered in bacteria and archea as an acquired immune response to protect the cells from infection. This technology has now evolved… read more here.
Keywords: plant genome; applications crispr; crispr cas9; technology ... See more keywords
Chromosome genomics uncovers plant genome organization and function.
Sign Up to like & getrecommendations! Published in 2020 at "Biotechnology advances"
DOI: 10.1016/j.biotechadv.2020.107659
Abstract: The identification of causal genomic loci and their interactions underlying various traits in plants has been greatly aided by progress in understanding the organization of the nuclear genome. This provides clues to the responses of… read more here.
Keywords: genomics uncovers; chromosome genomics; plant genome; uncovers plant ... See more keywords
Plant genome sequences: past, present, future.
Sign Up to like & getrecommendations! Published in 2019 at "Current opinion in plant biology"
DOI: 10.1016/j.pbi.2018.11.001
Abstract: The green plants (Viridiplantae) are an essential kingdom of life, responsible via photosynthesis for the majority of global primary production, and directly utilized by humankind for nutrition, animal feed, fuel, clothing, medicine and other purposes.… read more here.
Keywords: plant; genome sequences; sequences past; plant genome ... See more keywords
Twenty years of plant genome sequencing: achievements and challenges.
Sign Up to like & getrecommendations! Published in 2021 at "Trends in plant science"
DOI: 10.1016/j.tplants.2021.10.006
Abstract: Publication of the complete genome sequence of Arabidopsis thaliana, the first plant reference genome, in December 2000 heralded the beginning of the plant genome era. Over the past 20 years reference genomes have been generated… read more here.
Keywords: plant; twenty years; plant genome; genome sequencing ... See more keywords
SpCas9-NG self-targets the sgRNA sequence in plant genome editing
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DOI: 10.1038/s41477-020-0603-9
Abstract: Streptococcus pyogenes Cas9 (SpCas9)-NG recognizes NGN protospacer adjacent motifs and expands the scope of genome-editing tools. In this study, we found that SpCas9-NG not only targeted the genome but also efficiently self-targeted the single-guide RNA… read more here.
Keywords: genome editing; single guide; plant genome; genome ... See more keywords
Genome editing in plants using the compact editor CasΦ
Sign Up to like & getrecommendations! Published in 2022 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2216822120
Abstract: CRISPR-Cas systems have been developed as important tools for plant genome engineering. Here, we demonstrate that the hypercompact CasΦ nuclease is able to generate stably inherited gene edits in Arabidopsis, and that CasΦ guide RNAs… read more here.
Keywords: genome editing; cas; plant genome; hypercompact ... See more keywords
Kozak Sequence Acts as a Negative Regulator for De Novo Transcription Initiation of Newborn Coding Sequences in the Plant Genome
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DOI: 10.1093/molbev/msab069
Abstract: Abstract The manner in which newborn coding sequences and their transcriptional competency emerge during the process of gene evolution remains unclear. Here, we experimentally simulated eukaryotic gene origination processes by mimicking horizontal gene transfer events… read more here.
Keywords: plant; newborn coding; plant genome; gene ... See more keywords
The Rhododendron Plant Genome Database (RPGD): a comprehensive online omics database for Rhododendron
Sign Up to like & getrecommendations! Published in 2021 at "BMC Genomics"
DOI: 10.1186/s12864-021-07704-0
Abstract: Background The genus Rhododendron L . has been widely cultivated for hundreds of years around the world. Members of this genus are known for great ornamental and medicinal value. Owing to advances in sequencing technology,… read more here.
Keywords: rpgd; rhododendron plant; plant genome; database ... See more keywords
Fern Spores—“Ready-to-Use” Standards for Plant Genome Size Estimation Using a Flow Cytometric Approach
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DOI: 10.3390/plants12010140
Abstract: Spores and pollen of plants were used as flow cytometric materials to efficiently infer genome sizes. Given this advantage, they hold great potential for various flow cytometric applications, particularly as plant genome size standards. To… read more here.
Keywords: flow cytometric; ready use; spore; plant genome ... See more keywords