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Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1795-3_12
Abstract: In planta gene expression analysis and GFP-based confocal microscopy are two powerful techniques that may be coupled to assess the extent and dynamics of plant colonization by a fungal pathogen. Here we describe methods to…
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Keywords:
fusarium oxysporum;
microscopy;
gene expression;
colonization ... See more keywords
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Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7003-2_1
Abstract: The recent emergence of the CRISPR/Cas system has boosted the possibilities for precise genome engineering approaches throughout all kingdoms of life. The most common application for plants is targeted mutagenesis, whereby a Cas9-mediated DNA double-strand…
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Keywords:
gene targeting;
cas mediated;
crispr cas;
planta gene ... See more keywords