Enhancing the production of adeno‐associated virus (AAV)2 and AAV9 with high full capsid ratio in HEK293 cells through design‐of‐experiment optimization of triple plasmid ratio
Sign Up to like & getrecommendations! Published in 2024 at "Biotechnology Journal"
DOI: 10.1002/biot.202300667
Abstract: The recombinant adeno‐associated virus (rAAV) vectors used in gene therapy are usually produced by transfecting three different plasmids (Adenoviral helper plasmid (pHelper), AAV rep/cap plasmids (pRepCap), and Transgene plasmid (pAAV‐GOI)) into human embryonic kidney 293… read more here.
Keywords: capsid; hek293 cells; plasmid ratio; plasmid ... See more keywords
A platform method for plasmid isoforms analysis by capillary gel electrophoresis
Sign Up to like & getrecommendations! Published in 2022 at "ELECTROPHORESIS"
DOI: 10.1002/elps.202100343
Abstract: In the production of novel biological products, plasmids are often engineered into delivery vectors for target genes, which can be used directly as vaccines or as intermediate products for gene/cell therapy. Plasmid DNA exists in… read more here.
Keywords: gel electrophoresis; plasmid; platform method; method ... See more keywords
Plasmid Typing and Classification.
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DOI: 10.1007/978-1-4939-9877-7_22
Abstract: Plasmids identification and classification is an essential parameter in current bacterial typing. The most widely used PCR-based methods are the PCR-based replicon typing (PBRT) and the degenerate primer MOB typing (DPMT). PBRT targets the replicons… read more here.
Keywords: classification; typing classification; plasmid typing; biology ... See more keywords
Characterization and Abundance of Plasmid-Dependent Alphatectivirus Bacteriophages
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DOI: 10.1007/s00248-024-02401-3
Abstract: Antimicrobial resistance (AMR) is a major public health threat, exacerbated by the ability of bacteria to rapidly disseminate antimicrobial resistance genes (ARG). Since conjugative plasmids of the incompatibility group P (IncP) are ubiquitous mobile genetic… read more here.
Keywords: alphatectivirus; conjugative plasmids; culture; plasmid ... See more keywords
Development of a shuttle plasmid without host restriction sites for efficient transformation and heterologous gene expression in Clostridium cellulovorans
Sign Up to like & getrecommendations! Published in 2019 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-019-09882-0
Abstract: Clostridium cellulovorans capable of producing large amounts of acetate and butyrate from cellulose is a promising candidate for biofuels and biochemicals production from lignocellulosic biomass. However, the restriction modification (RM) systems of C. cellulovorans hindered… read more here.
Keywords: shuttle plasmid; plasmid without; clostridium cellulovorans; plasmid ... See more keywords
Characterization of a Cryptic Rolling-Circle Replication Plasmid pMK8 from Enterococcus durans 1–8
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DOI: 10.1007/s00284-018-1509-x
Abstract: A novel cryptic plasmid from Enterococcus durans 1–8, designated as pMK8, was sequenced and analyzed in this study. It consists of 3337 bp with a G + C content of 33.11%. Sequence analysis of pMK8 revealed three putative… read more here.
Keywords: pmk8; enterococcus durans; replication; plasmid ... See more keywords
Production of lentiviral vectors in suspension cells using low proportion of supercoiled circular plasmid DNA
Sign Up to like & getrecommendations! Published in 2020 at "Cytotechnology"
DOI: 10.1007/s10616-020-00433-4
Abstract: The supercoiled circular (SC) topology form of plasmid DNA has been regarded to be advantageous over open circular or linearized analogue in transfection and expression efficiency, and therefore are largely demanded in the biopharmaceutical manufacturing.… read more here.
Keywords: lentiviral vectors; plasmid dna; plasmid; proportion plasmid ... See more keywords
Dual Transmission Dynamics of Carbapenem Resistance in Pediatric Oncology: Plasmid-Mediated and Clonal Spread of blaNDM-5 Enterobacterales.
Sign Up to like & getrecommendations! Published in 2025 at "Infectious diseases and therapy"
DOI: 10.1007/s40121-025-01254-8
Abstract: Carbapenem-resistant Enterobacterales (CRE) pose a major threat to immunocompromised pediatric oncology patients. However, the routes of resistance spread in this vulnerable population remain poorly understood, despite their importance for guiding infection control. We analyzed 189… read more here.
Keywords: oncology; resistance; dynamics carbapenem; pediatric oncology ... See more keywords
Engineering the bacterium Comamonas testosteroni CNB-1: Plasmid curing and genetic manipulation
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DOI: 10.1016/j.bej.2018.01.030
Abstract: Abstract Comamonas species are attractive microbial hosts for bioremediation engineering due to their versatile metabolic capacity and diverse niches inhabited. To establish Comamonas testosteroni CNB-1—a common Comamonas strain—as a programmable cellular chassis, its indigenous 91-kb-long… read more here.
Keywords: testosteroni cnb; engineering; comamonas testosteroni; plasmid ... See more keywords
Interaction of pDNA with reverse phase chitosome
Sign Up to like & getrecommendations! Published in 2018 at "Colloids and Surfaces A: Physicochemical and Engineering Aspects"
DOI: 10.1016/j.colsurfa.2018.02.005
Abstract: Abstract The use of liposomes and polyelectrolytes is on scientific and technologic scrutiny for development of gene nano carriers. We produced a reverse phase lipid:chitosan “chitosome” to which plasmid DNA (pDNA) effectively interacts and compacts.… read more here.
Keywords: interaction pdna; reverse phase; plasmid; pdna ... See more keywords
Conjugative IncHI2 plasmid harboring novel class 1 integron mediated dissemination of multidrug resistance genes in Salmonella Typhimurium
Sign Up to like & getrecommendations! Published in 2021 at "Food Control"
DOI: 10.1016/j.foodcont.2020.107810
Abstract: Abstract Salmonella enterica serovar Typhimurium is one of the most important serovars among nontyphoidal Salmonella. The inappropriate and over usage of antimicrobials in clinical and animal husbandry has increased the prevalence of antimicrobial-resistant Salmonella Typhimurium… read more here.
Keywords: class integron; typhimurium; resistance; dissemination ... See more keywords