A platform method for plasmid isoforms analysis by capillary gel electrophoresis
Sign Up to like & getrecommendations! Published in 2022 at "ELECTROPHORESIS"
DOI: 10.1002/elps.202100343
Abstract: In the production of novel biological products, plasmids are often engineered into delivery vectors for target genes, which can be used directly as vaccines or as intermediate products for gene/cell therapy. Plasmid DNA exists in… read more here.
Keywords: gel electrophoresis; plasmid; platform method; method ... See more keywords
Plasmid Typing and Classification.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9877-7_22
Abstract: Plasmids identification and classification is an essential parameter in current bacterial typing. The most widely used PCR-based methods are the PCR-based replicon typing (PBRT) and the degenerate primer MOB typing (DPMT). PBRT targets the replicons… read more here.
Keywords: classification; typing classification; plasmid typing; biology ... See more keywords
Development of a shuttle plasmid without host restriction sites for efficient transformation and heterologous gene expression in Clostridium cellulovorans
Sign Up to like & getrecommendations! Published in 2019 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-019-09882-0
Abstract: Clostridium cellulovorans capable of producing large amounts of acetate and butyrate from cellulose is a promising candidate for biofuels and biochemicals production from lignocellulosic biomass. However, the restriction modification (RM) systems of C. cellulovorans hindered… read more here.
Keywords: shuttle plasmid; plasmid without; clostridium cellulovorans; plasmid ... See more keywords
Characterization of a Cryptic Rolling-Circle Replication Plasmid pMK8 from Enterococcus durans 1–8
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DOI: 10.1007/s00284-018-1509-x
Abstract: A novel cryptic plasmid from Enterococcus durans 1–8, designated as pMK8, was sequenced and analyzed in this study. It consists of 3337 bp with a G + C content of 33.11%. Sequence analysis of pMK8 revealed three putative… read more here.
Keywords: pmk8; enterococcus durans; replication; plasmid ... See more keywords
Production of lentiviral vectors in suspension cells using low proportion of supercoiled circular plasmid DNA
Sign Up to like & getrecommendations! Published in 2020 at "Cytotechnology"
DOI: 10.1007/s10616-020-00433-4
Abstract: The supercoiled circular (SC) topology form of plasmid DNA has been regarded to be advantageous over open circular or linearized analogue in transfection and expression efficiency, and therefore are largely demanded in the biopharmaceutical manufacturing.… read more here.
Keywords: lentiviral vectors; plasmid dna; plasmid; proportion plasmid ... See more keywords
Engineering the bacterium Comamonas testosteroni CNB-1: Plasmid curing and genetic manipulation
Sign Up to like & getrecommendations! Published in 2018 at "Biochemical Engineering Journal"
DOI: 10.1016/j.bej.2018.01.030
Abstract: Abstract Comamonas species are attractive microbial hosts for bioremediation engineering due to their versatile metabolic capacity and diverse niches inhabited. To establish Comamonas testosteroni CNB-1—a common Comamonas strain—as a programmable cellular chassis, its indigenous 91-kb-long… read more here.
Keywords: testosteroni cnb; engineering; comamonas testosteroni; plasmid ... See more keywords
Interaction of pDNA with reverse phase chitosome
Sign Up to like & getrecommendations! Published in 2018 at "Colloids and Surfaces A: Physicochemical and Engineering Aspects"
DOI: 10.1016/j.colsurfa.2018.02.005
Abstract: Abstract The use of liposomes and polyelectrolytes is on scientific and technologic scrutiny for development of gene nano carriers. We produced a reverse phase lipid:chitosan “chitosome” to which plasmid DNA (pDNA) effectively interacts and compacts.… read more here.
Keywords: interaction pdna; reverse phase; plasmid; pdna ... See more keywords
Conjugative IncHI2 plasmid harboring novel class 1 integron mediated dissemination of multidrug resistance genes in Salmonella Typhimurium
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DOI: 10.1016/j.foodcont.2020.107810
Abstract: Abstract Salmonella enterica serovar Typhimurium is one of the most important serovars among nontyphoidal Salmonella. The inappropriate and over usage of antimicrobials in clinical and animal husbandry has increased the prevalence of antimicrobial-resistant Salmonella Typhimurium… read more here.
Keywords: class integron; typhimurium; resistance; dissemination ... See more keywords
Characterization of a novel theta-type plasmid pSM409 of Enterococcus faecium RME isolated from raw milk.
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DOI: 10.1016/j.gene.2021.145459
Abstract: Enterococcal plasmids have generated renewed interest for their indispensable role in pathogenesis and dissemination of multidrug-resistance. Recently, a novel plasmid pSM409 (4303-bp, GC% = 33.6 %), devoid of antibiotic-resistance and virulence genes, has been identified… read more here.
Keywords: plasmid; theta type; faecium rme; psm409 ... See more keywords
An unusual occurrence of plasmid-mediated blaOXA-23 carbapenemase in clinical isolates of Escherichia coli from India.
Sign Up to like & getrecommendations! Published in 2017 at "International journal of antimicrobial agents"
DOI: 10.1016/j.ijantimicag.2017.01.012
Abstract: The blaOXA-23 group was considered as the first group of OXA-type β-lactamases conferring carbapenem resistance and has been reported worldwide in Acinetobacter baumannii, however their presence in Escherichia coli is very rare and unique. This… read more here.
Keywords: unusual occurrence; escherichia coli; clinical isolates; blaoxa ... See more keywords
Genome analysis of antimicrobial resistance, virulence, and plasmid presence in Turkish Salmonella serovar Infantis isolates.
Sign Up to like & getrecommendations! Published in 2019 at "International journal of food microbiology"
DOI: 10.1016/j.ijfoodmicro.2019.108275
Abstract: Salmonella enterica subsp. enterica serovar Infantis (S. Infantis) isolates were found to have a multi-drug resistance profile (kanamycin, streptomycin, nalidixic acid, tetracycline, sulfonamide, and sometimes to ampicillin) and high prevalence (91%) in Turkish poultry in… read more here.
Keywords: infantis; analysis; infantis isolates; antimicrobial resistance ... See more keywords