Analytical separation of plasmid DNA isoforms using anion exchanging chromatographic monoliths with 6 µm channels.
Sign Up to like & getrecommendations! Published in 2023 at "Electrophoresis"
DOI: 10.1002/elps.202300031
Abstract: High-performance liquid chromatography (HPLC)-based analytical assays are used to effectively monitor purity and quantity of plasmid DNA (pDNA) throughout the purification process. However, the phenomenon of physical entrapment of open circular (OC) isoforms pDNA inside… read more here.
Keywords: plasmid dna; anion exchanging; analytical separation; pdna ... See more keywords
Transfection reagent artefact likely accounts for some reports of extracellular vesicle function
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Extracellular Vesicles"
DOI: 10.1002/jev2.12253
Abstract: Abstract Extracellular vesicles (EV) are important mediators of cell communication and physiology. EVs are frequently investigated by transiently transfecting cells with plasmid DNA to produce EVs modified with protein(s) or nucleic acid(s) of interest. DNA‐transfection… read more here.
Keywords: function; dna; plasmid dna; transfection reagent ... See more keywords
Lipofection of plasmid DNA into human mast cell lines using lipid nanoparticles generated by microfluidic mixing
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Leukocyte Biology"
DOI: 10.1002/jlb.3ta0517-192r
Abstract: Mast cells are important immune cells that have significant roles in mediating allergy and asthma. Therefore, studying the molecular mechanisms regulating these and other processes in mast cells is important to elucidate. Methods such as… read more here.
Keywords: lipofection; cell lines; mast; plasmid dna ... See more keywords
Systematic study of enzymatic degradation and plasmid DNA complexation of mucus penetrating star-shaped lysine/sarcosine polypept(o)ides with different block arrangements.
Sign Up to like & getrecommendations! Published in 2022 at "Macromolecular bioscience"
DOI: 10.1002/mabi.202200175
Abstract: 8-Arm star polypep(o)ides comprising cationic polylysine and hydrophilic polysarcosine blocks with a degree of polymerization of 30 per block are synthesized. Two different block sequences with polylysine as the inner and polysarcosine as the outer… read more here.
Keywords: star; plasmid dna; different block; mucus ... See more keywords
Extraction and Analysis of Plasmid DNA from Listeria monocytogenes.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0982-8_12
Abstract: A plasmid preparation is a method used to extract and purify plasmid DNA. Methods developed to purify plasmid DNA from bacteria generally involve harvesting and alkaline lysis of the bacteria, precipitation of chromosomal DNA and… read more here.
Keywords: plasmid dna; extraction analysis; listeria monocytogenes; dna ... See more keywords
Study of the Efficiency of the Hydroporation for Delivery of Plasmid DNA to the Cells on the Model of Toxic Neuropathy
Sign Up to like & getrecommendations! Published in 2018 at "Bulletin of Experimental Biology and Medicine"
DOI: 10.1007/s10517-018-4083-0
Abstract: We compared the efficiency of delivery of plasmid DNA (active ingredient concentration 1 mg/kg) that provides production of nerve growth factor (NGF) after intravenous administration to rats and after administration by hydroporation. The method of… read more here.
Keywords: hydroporation; administration; dna; plasmid dna ... See more keywords
Production of lentiviral vectors in suspension cells using low proportion of supercoiled circular plasmid DNA
Sign Up to like & getrecommendations! Published in 2020 at "Cytotechnology"
DOI: 10.1007/s10616-020-00433-4
Abstract: The supercoiled circular (SC) topology form of plasmid DNA has been regarded to be advantageous over open circular or linearized analogue in transfection and expression efficiency, and therefore are largely demanded in the biopharmaceutical manufacturing.… read more here.
Keywords: lentiviral vectors; plasmid dna; plasmid; proportion plasmid ... See more keywords
Recombinant Plasmid DNA Construct Encoding Combination of vegf165 and bmp2 cDNAs Stimulates Osteogenesis and Angiogenesis In Vitro
Sign Up to like & getrecommendations! Published in 2017 at "BioNanoScience"
DOI: 10.1007/s12668-016-0300-3
Abstract: Though normally bone regenerates well, non-union, delayed union, or defective bone formation can occur. Since impaired healing is often caused by local trophic disorders, standard fracture management is often ineffective. Gene therapy is a promising… read more here.
Keywords: construct encoding; dna construct; bmp2; plasmid dna ... See more keywords
Detection of luxA Gene on Plasmid DNA of Luminous Bacteria Vibrio campbellii STF1
Sign Up to like & getrecommendations! Published in 2020 at "National Academy Science Letters"
DOI: 10.1007/s40009-020-00952-w
Abstract: Bacterial bioluminescence is emitted due to regulation by lux genes present on the chromosomal DNA. Earlier studies have attempted to find possible occurrence of lux genes on plasmid DNA (pDNA) but did not find such… read more here.
Keywords: gene plasmid; luxa gene; plasmid dna; dna luminous ... See more keywords
Development of an adaptive electroporation system for intratumoral plasmid DNA delivery.
Sign Up to like & getrecommendations! Published in 2018 at "Bioelectrochemistry"
DOI: 10.1016/j.bioelechem.2018.04.005
Abstract: Intratumoral electroporation of plasmid DNA encoding the proinflammatory cytokine interleukin 12 promotes innate and adaptive immune responses correlating with anti-tumor effects. Clinical electroporation conditions are fixed parameters optimized in preclinical tumors, which consist of cells… read more here.
Keywords: development adaptive; electroporation; adaptive electroporation; plasmid dna ... See more keywords
Injectable nanofibrous spongy microspheres for NR4A1 plasmid DNA transfection to reverse fibrotic degeneration and support disc regeneration.
Sign Up to like & getrecommendations! Published in 2017 at "Biomaterials"
DOI: 10.1016/j.biomaterials.2017.03.029
Abstract: Safe and efficient gene therapy is highly desired for controlling pathogenic fibrosis of nucleus pulposus (NP) tissue, which would result in intervertebral disc (IVD) degeneration and disability if left untreated. In this work, a hyperbranched… read more here.
Keywords: fibrosis; plasmid dna; spongy microspheres; nanofibrous spongy ... See more keywords