Efficient strategies to enhance plasmid stability for fermentation of recombinant Escherichia coli harboring tyrosine phenol lyase.
Sign Up to like & getrecommendations! Published in 2021 at "Biotechnology letters"
DOI: 10.1007/s10529-021-03082-5
Abstract: OBJECTIVE To solve the bottleneck of plasmid instability during microbial fermentation of L-DOPA with recombinant Escherichia coli expressing heterologous tyrosine phenol lyase. RESULTS The tyrosine phenol lyase from Fusobacterium nucleatum was constitutively expressed in E.… read more here.
Keywords: plasmid stability; phenol lyase; fermentation; tyrosine phenol ... See more keywords
Emergence of plasmid stability under non-selective conditions maintains antibiotic resistance
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DOI: 10.1038/s41467-019-10600-7
Abstract: Plasmid acquisition is an important mechanism of rapid adaptation and niche expansion in prokaryotes. Positive selection for plasmid-coded functions is a major driver of plasmid evolution, while plasmids that do not confer a selective advantage… read more here.
Keywords: stability non; plasmid stability; non selective; resistance ... See more keywords
Host-specific plasmid evolution explains the variable spread of clinical antibiotic-resistance plasmids
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DOI: 10.1101/2022.07.06.498992
Abstract: Antibiotic resistance encoded on plasmids is a pressing global health problem. Predicting which plasmids spread/decline in the long term remains a huge challenge, even though some key parameters influencing plasmid stability have been identified, such… read more here.
Keywords: antibiotic resistance; plasmid stability; plasmid; bacterium plasmid ... See more keywords
Use and Evaluation of a pES213-Derived Plasmid for the Constitutive Expression of gfp Protein in Pathogenic Vibrios: a Tagging Tool for In Vitro Studies
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DOI: 10.1128/spectrum.02490-22
Abstract: Prior research has suggested that the use of Aliivibrio fischeri-derived donor plasmids with the pES213 origin of replication may provide increased plasmid stability for the tagging of vibrios compared to Escherichia coli-derived p15A plasmids. Here,… read more here.
Keywords: pes213 derived; plasmid stability; derived plasmid;