Rubisco Engineering by Plastid Transformation and Protocols for Assessing Expression.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1472-3_10
Abstract: The assimilation of CO2 within chloroplasts is catalyzed by the bifunctional enzyme ribulose-1,5-bisphosphate carboxylase/oxygenase, Rubisco. Within higher plants the Rubisco large subunit gene, rbcL, is encoded in the plastid genome, while the Rubisco small subunit… read more here.
Keywords: plastid transformation; rubisco engineering; engineering plastid; rubisco ... See more keywords
Plastid transformation and its application in metabolic engineering.
Sign Up to like & getrecommendations! Published in 2018 at "Current opinion in biotechnology"
DOI: 10.1016/j.copbio.2017.07.004
Abstract: Metabolic pathway engineering by transgene expression from the plastid (chloroplast) genome offers significant attractions, including straightforward multigene engineering by pathway expression from operons, high transgene expression levels, and increased transgene containment due to maternal inheritance… read more here.
Keywords: transformation application; engineering; metabolic engineering; plastid ... See more keywords
Plastid transformation: Advances and challenges for its implementation in agricultural crops
Sign Up to like & getrecommendations! Published in 2021 at "Electronic Journal of Biotechnology"
DOI: 10.1016/j.ejbt.2021.03.005
Abstract: Abstract Chloroplast biotechnology has emerged as a promissory platform for the development of modified plants to express products aimed mainly at the pharmaceutical, agricultural, and energy industries. This technology's high value is due to its… read more here.
Keywords: implementation agricultural; plastid transformation; transformation advances; advances challenges ... See more keywords
A simple technology for plastid transformation with fragmented DNA.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of experimental botany"
DOI: 10.1093/jxb/erac256
Abstract: Plastid engineering has several unique advantages such as high expression of transgene due to high polyploid of plastid genome and environmental biosafety because of maternal inheritance of transgene, and becomes a promising tool for molecular… read more here.
Keywords: plastid genome; dna; technology; plastid transformation ... See more keywords
Functional peptide-mediated plastid transformation in tobacco, rice, and kenaf
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Plant Science"
DOI: 10.3389/fpls.2022.989310
Abstract: In plant engineering, plastid transformation is more advantageous than nuclear transformation because it results in high levels of protein expression from multiple genome copies per cell and is unaffected by gene silencing. The common plastid… read more here.
Keywords: dna; tobacco rice; plastid transformation; rice kenaf ... See more keywords
Plastid Transformation: New Challenges in the Circular Economy Era
Sign Up to like & getrecommendations! Published in 2022 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms232315254
Abstract: In a circular economy era the transition towards renewable and sustainable materials is very urgent. The development of bio-based solutions, that can ensure technological circularity in many priority areas (e.g., agriculture, biotechnology, ecology, green industry,… read more here.
Keywords: plastid transformation; transformation; economy era; circular economy ... See more keywords