A Nucleophilic Activity‐Based Probe Enables Profiling of PLP‐Dependent Enzymes
Sign Up to like & getrecommendations! Published in 2023 at "ChemBioChem"
DOI: 10.1002/cbic.202200669
Abstract: PLP‐dependent enzymes represent an important class of highly “druggable” enzymes that perform a wide array of critical reactions to support all organisms. Inhibition of individual members of this family of enzymes has been validated as… read more here.
Keywords: dependent enzymes; plp dependent; nucleophilic activity; activity based ... See more keywords
A single amino acid substitution converts a histidine decarboxylase to an imidazole acetaldehyde synthase.
Sign Up to like & getrecommendations! Published in 2020 at "Archives of biochemistry and biophysics"
DOI: 10.1016/j.abb.2020.108551
Abstract: Histidine decarboxylase (HDC; EC 4.1.1.22), an enzyme that catalyzes histamine synthesis with high substrate specificity, is a member of the group II pyridoxal 5'-phosphate (PLP) -dependent decarboxylase family. Tyrosine is a conserved residue among group… read more here.
Keywords: histidine decarboxylase; decarboxylase; amino acid; acetaldehyde synthase ... See more keywords
A Fold Type II PLP-Dependent Enzyme from Fusobacterium nucleatum Functions as a Serine Synthase and Cysteine Synthase.
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DOI: 10.1021/acs.biochem.0c00902
Abstract: Serine synthase (SS) from Fusobacterium nucleatum is a fold type II pyridoxal 5'-phosphate (PLP)-dependent enzyme that catalyzes the β-replacement of l-cysteine with water to form l-serine and H2S. Herein, we show that SS can also… read more here.
Keywords: synthase; cysteine; fold type; serine synthase ... See more keywords
Catalytic Roles of Coenzyme Pyridoxal-5'-phosphate (PLP) in PLP-dependent Enzymes: Reaction Pathway for Methionine-γ-lyase-catalyzed L-methionine Depletion.
Sign Up to like & getrecommendations! Published in 2020 at "ACS catalysis"
DOI: 10.1021/acscatal.9b03907
Abstract: Pyridoxal-5'-phosphate (PLP), the active form of vitamin B6, is an important and versatile coenzyme involved in a variety of enzymatic reactions, accounting for about 4% of all classified activities. However, the detailed catalytic reaction pathways… read more here.
Keywords: plp; phosphate plp; pyridoxal phosphate; dependent enzymes ... See more keywords
Snapshots of the Catalytic Cycle of an O2, Pyridoxal Phosphate-Dependent Hydroxylase.
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DOI: 10.1021/acschembio.8b00039
Abstract: Enzymes that catalyze hydroxylation of unactivated carbons normally contain heme and nonheme iron cofactors. By contrast, how a pyridoxal phosphate (PLP)-dependent enzyme could catalyze such a hydroxylation was unknown. Here, we investigate RohP, a PLP-dependent… read more here.
Keywords: snapshots catalytic; plp dependent; pyridoxal phosphate; catalytic cycle ... See more keywords