Concise Review: Signaling Control of Early Fate Decisions Around the Human Pluripotent Stem Cell State
Sign Up to like & getrecommendations! Published in 2017 at "STEM CELLS"
DOI: 10.1002/stem.2527
Abstract: Human embryonic stem cells (hESCs) present a fascinating and powerful system for generating specialized cell types of the human body. Culture and directed differentiation of these cells however requires an understanding of the pluripotent ground… read more here.
Keywords: state; stem cells; pluripotent; fate ... See more keywords
Optimization of Differentiation of Nonhuman Primate Pluripotent Cells Using a Combinatorial Approach.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9007-8_14
Abstract: The directed differentiation of pluripotent stem cells to a desired lineage often involves complex and lengthy protocols. In order to study the requirements for differentiation in a systematic way, we present here methodology for an… read more here.
Keywords: pluripotent; differentiation nonhuman; differentiation; nonhuman primate ... See more keywords
ERK-independent African Green monkey pluripotent stem cells in a putative chimera-competent state.
Sign Up to like & getrecommendations! Published in 2019 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2019.01.037
Abstract: Generating human organs inside interspecies chimeras might one day produce patient-specific organs for clinical applications, but further advances in identifying human chimera-competent pluripotent stem (PS) cells are needed. Moreover, the potential for human PS cells… read more here.
Keywords: chimera competent; stem cells; chimera; pluripotent ... See more keywords
A semi-interpenetrating network of polyacrylamide and recombinant basement membrane allows pluripotent cell culture in a soft, ligand-rich microenvironment.
Sign Up to like & getrecommendations! Published in 2017 at "Biomaterials"
DOI: 10.1016/j.biomaterials.2016.12.005
Abstract: The physical properties of the extracellular matrix play an essential role in guiding stem cell differentiation and tissue morphogenesis both in vivo and in vitro. Existing work to investigate the role of matrix mechanics in directing stem… read more here.
Keywords: pluripotent; basement membrane; polyacrylamide recombinant; recombinant basement ... See more keywords
Transcriptional Heterogeneity in Naive and Primed Human Pluripotent Stem Cells at Single-Cell Resolution
Sign Up to like & getrecommendations! Published in 2019 at "Cell Reports"
DOI: 10.1016/j.celrep.2018.12.099
Abstract: Summary Conventional human embryonic stem cells are considered to be primed pluripotent but can be induced to enter a naive state. However, the transcriptional features associated with naive and primed pluripotency are still not fully… read more here.
Keywords: single cell; cell; pluripotent; naive primed ... See more keywords
Pluripotent cells display enhanced resistance to mutagenesis.
Sign Up to like & getrecommendations! Published in 2017 at "Stem cell research"
DOI: 10.1016/j.scr.2016.12.029
Abstract: Pluripotent cells have been reported to exhibit lower frequencies of point mutations and higher levels of DNA repair than differentiated cells. This predicts that pluripotent cells are less susceptible to mutagenic exposures than differentiated cells.… read more here.
Keywords: pluripotent cells; point mutations; pluripotent; cells display ... See more keywords
Agarose microgel culture delineates lumenogenesis in naive and primed human pluripotent stem cells
Sign Up to like & getrecommendations! Published in 2021 at "Stem Cell Reports"
DOI: 10.1016/j.stemcr.2021.04.009
Abstract: Summary Human periimplantation development requires the transformation of the naive pluripotent epiblast into a polarized epithelium. Lumenogenesis plays a critical role in this process, as the epiblast undergoes rosette formation and lumen expansion to form… read more here.
Keywords: human pluripotent; pluripotent; pluripotent stem; lumenogenesis naive ... See more keywords
Naive Pluripotent and Trophoblastic Stem Cell Lines as a Model for Detecting Missing Proteins in the Context of the Chromosome-Centric Human Proteome Project.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of proteome research"
DOI: 10.1021/acs.jproteome.2c00496
Abstract: The Chromosome-centric Human Proteome Project (C-HPP) aims at identifying the proteins as gene products encoded by the human genome, characterizing their isoforms and functions. The existence of products has now been confirmed for 93.2% of… read more here.
Keywords: missing proteins; chromosome centric; pluripotent; centric human ... See more keywords
Widespread reorganisation of pluripotent factor binding and gene regulatory interactions between human pluripotent states
Sign Up to like & getrecommendations! Published in 2021 at "Nature Communications"
DOI: 10.1038/s41467-021-22201-4
Abstract: The transition from naive to primed pluripotency is accompanied by an extensive reorganisation of transcriptional and epigenetic programmes. However, the role of transcriptional enhancers and three-dimensional chromatin organisation in coordinating these developmental programmes remains incompletely… read more here.
Keywords: human pluripotent; pluripotent states; pluripotent; gene regulatory ... See more keywords
Organization of the Pluripotent Genome.
Sign Up to like & getrecommendations! Published in 2020 at "Cold Spring Harbor perspectives in biology"
DOI: 10.1101/cshperspect.a040204
Abstract: In the past several decades, the establishment of in vitro models of pluripotency has ushered in a golden era for developmental and stem cell biology. Research in this arena has led to profound insights into… read more here.
Keywords: pluripotent; pluripotent genome; biology; organization pluripotent ... See more keywords
Abstract 476: Pharmacogenetics and Drug Discovery for Anthracycline-Induced Cardiotoxicity Enabled by Sinoatrial Node-like Cells Derived From Human Pluripotent Stem Cells
Sign Up to like & getrecommendations! Published in 2019 at "Circulation Research"
DOI: 10.1161/res.125.suppl_1.476
Abstract: The sinoatrial node (SAN) is the primary pacemaker of the heart. The human SAN is poorly understood due to limited primary tissue access and lack of robust in vitro derivation methods. We developed an efficient… read more here.
Keywords: sinoatrial node; human pluripotent; pluripotent; pluripotent stem ... See more keywords