Feeder cells treated with ethanol can be used to maintain self‐renewal and pluripotency of human pluripotent stem cells
Sign Up to like & getrecommendations! Published in 2022 at "FEBS Open Bio"
DOI: 10.1002/2211-5463.13538
Abstract: Feeder cells play an important role in the culture of human pluripotent stem cells (hPSCs) in vitro. Previously, we used methanol as a fixative to prepare feeder cells for the cultivation of pluripotent stem cells (PSCs),… read more here.
Keywords: pluripotent stem; self renewal; feeder cells; cells treated ... See more keywords
Engineering of Mature Human Induced Pluripotent Stem Cell‐Derived Cardiomyocytes Using Substrates with Multiscale Topography
Sign Up to like & getrecommendations! Published in 2018 at "Advanced Functional Materials"
DOI: 10.1002/adfm.201707378
Abstract: Producing mature and functional cardiomyocytes (CMs) by in vitro differentiation of induced pluripotent stem cells (iPSCs) using only biochemical cues is challenging. To mimic the biophysical and biomechanical complexity of the native in vivo environment… read more here.
Keywords: topography; mature; pluripotent stem; induced pluripotent ... See more keywords
Thermoresponsive Hydrogels as Microniches for Growth and Controlled Release of Induced Pluripotent Stem Cells
Sign Up to like & getrecommendations! Published in 2021 at "Advanced Functional Materials"
DOI: 10.1002/adfm.202010630
Abstract: The recently emerging stem‐cell artificial niche engineering in induced pluripotent stem cell (iPSCs) 3D cultures has provided enormous opportunities to fully utilize the potential of these cells in biomedical applications. Although a fully chemically defined… read more here.
Keywords: controlled release; niche; induced pluripotent; pluripotent stem ... See more keywords
Investigating and Resolving Cardiotoxicity Induced by COVID‐19 Treatments using Human Pluripotent Stem Cell‐Derived Cardiomyocytes and Engineered Heart Tissues
Sign Up to like & getrecommendations! Published in 2022 at "Advanced Science"
DOI: 10.1002/advs.202203388
Abstract: Coronavirus disease 2019 continues to spread worldwide. Given the urgent need for effective treatments, many clinical trials are ongoing through repurposing approved drugs. However, clinical data regarding the cardiotoxicity of these drugs are limited. Human… read more here.
Keywords: cell; stem cell; pluripotent stem; human pluripotent ... See more keywords
Symmetry Breaking of Human Pluripotent Stem Cells (hPSCs) in Micropattern Generates a Polarized Spinal Cord-Like Organoid (pSCO) with Dorsoventral Organization.
Sign Up to like & getrecommendations! Published in 2023 at "Advanced science"
DOI: 10.1002/advs.202301787
Abstract: Axis formation and related spatial patterning are initiated by symmetry breaking during development. A geometrically confined culture of human pluripotent stem cells (hPSCs) mimics symmetry breaking and cell patterning. Using this, polarized spinal cord organoids… read more here.
Keywords: pluripotent stem; human pluripotent; spinal cord; symmetry breaking ... See more keywords
Optimized protocol for the hepatic differentiation of induced pluripotent stem cells in a fluidic microenvironment
Sign Up to like & getrecommendations! Published in 2019 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.26970
Abstract: In the present study, we evaluated the performance of different protocols for the hepatic differentiation of human‐induced pluripotent stem cells (hiPSCs) in microfluidic biochips. Strategies for complete and partial on‐chip differentiation were tested. Unlike full… read more here.
Keywords: hepatic differentiation; differentiation; pluripotent stem; induced pluripotent ... See more keywords
Micropatterned substrates with physiological stiffness promote cell maturation and Pompe disease phenotype in human induced pluripotent stem cell‐derived skeletal myocytes
Sign Up to like & getrecommendations! Published in 2019 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.27075
Abstract: Recent advances in bioengineering have enabled cell culture systems that more closely mimic the native cellular environment. Here, we demonstrated that human induced pluripotent stem cell (iPSC)‐derived myogenic progenitors formed highly‐aligned myotubes and contracted when… read more here.
Keywords: human induced; disease; stem cell; pluripotent stem ... See more keywords
Dissolved oxygen concentration regulates human hepatic organoid formation from pluripotent stem cells in a fully controlled bioreactor
Sign Up to like & getrecommendations! Published in 2020 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.27521
Abstract: Developing technologies for scalable production of human organoids has gained increased attention for “organoid medicine” and drug discovery. We developed a scalable and integrated differentiation process for generation of hepatic organoid from human pluripotent stem… read more here.
Keywords: hepatic organoid; oxygen concentration; pluripotent stem; dissolved oxygen ... See more keywords
Diphenyleneiodonium efficiently inhibits the characteristics of a cancer stem cell model derived from induced pluripotent stem cells
Sign Up to like & getrecommendations! Published in 2022 at "Cell Biochemistry and Function"
DOI: 10.1002/cbf.3696
Abstract: Diphenyleneiodonium (DPI) has long been evaluated as an anticancer drug inhibiting NADPH oxidase, the IC50 in several cancer cell lines was reported 10 µM, which is too high for efficacy. In this study, we employed miPS‐Huh7cmP… read more here.
Keywords: stem cell; pluripotent stem; cancer stem; induced pluripotent ... See more keywords
Quantitative Assessment of Sialo‐Glycoproteins and N‐Glycans during Cardiomyogenic Differentiation of Human Induced Pluripotent Stem Cells
Sign Up to like & getrecommendations! Published in 2017 at "ChemBioChem"
DOI: 10.1002/cbic.201700100
Abstract: Human induced pluripotent stem‐cell‐derived cardiomyocytes (hiPSC CMs) may be used in regenerative medicine for individualized tissue transplants in the future. For application in patients, the generated CMs have to be highly pure and well characterized.… read more here.
Keywords: glycoproteins glycans; sialo glycoproteins; human induced; differentiation ... See more keywords
Chalcone Supported Cardiac Mesoderm Induction in Human Pluripotent Stem Cells for Heart Muscle Engineering.
Sign Up to like & getrecommendations! Published in 2021 at "ChemMedChem"
DOI: 10.1002/cmdc.202100222
Abstract: Human pluripotent stem cells (hPSCs) hold great promise for applications in cell therapy and drug screening in the cardiovascular field. Bone morphogenetic protein 4 (BMP4) is key for early cardiac mesoderm induction in hPSC and… read more here.
Keywords: cardiac mesoderm; mesoderm induction; human pluripotent; pluripotent stem ... See more keywords