Influence of the fixation/permeabilization step on peptide nucleic acid fluorescence in situ hybridization (PNA-FISH) for the detection of bacteria
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DOI: 10.1371/journal.pone.0196522
Abstract: Fluorescence in situ Hybridization (FISH) is a versatile, widespread and widely- used technique in microbiology. The first step of FISH—fixation/permeabilization—is crucial to the outcome of the method. This work aimed to systematically evaluate fixation/permeabilization protocols… read more here.
Keywords: fixation permeabilization; fluorescence situ; situ hybridization; permeabilization ... See more keywords