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   Articles with "pngase" as a keyword



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PNGase H + variant from Rudaea cellulosilytica with improved deglycosylation efficiency for rapid analysis of eukaryotic N‐glycans and hydrogen deuterium exchange mass spectrometry analysis of glycoproteins

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recommendations! Published in 2022 at "Rapid Communications in Mass Spectrometry"

DOI: 10.1002/rcm.9376

Abstract: The analysis of glycoproteins and the comparison of protein N‐glycosylation from different eukaryotic origins require unbiased and robust analytical workflows. The structural and functional analysis of vertebrate protein N‐glycosylation currently depends extensively on bacterial peptide‐N4‐(N‐acetyl‐β‐glucosaminyl)… read more here.

Keywords: mass spectrometry; analysis; analysis glycoproteins; pngase ... See more keywords
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Enhanced Recombinant Protein Production of Soluble, Highly Active and Immobilizable PNGase F

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recommendations! Published in 2022 at "Molecular Biotechnology"

DOI: 10.1007/s12033-022-00464-6

Abstract: High resolution analysis of N-glycans can be performed after their endoglycosidase mediated removal from proteins. N-glycosidase F peptide (PNGase F) is one the most frequently used enzyme for this purpose. Because of the significant demand… read more here.

Keywords: pngase; recombinant protein; protein production; production ... See more keywords
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Development of a colorimetric PNGase activity assay.

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recommendations! Published in 2019 at "Carbohydrate research"

DOI: 10.1016/j.carres.2018.11.007

Abstract: PNGases are crucial targets and valuable tools in analyzing asparagine-linked carbohydrate moieties (N-glycans) of glycoproteins. Activity tests of PNGases have been little improved since their discovery four decades ago, and still rely on observing deglycosylation… read more here.

Keywords: colorimetric pngase; activity assay; development colorimetric; pngase ... See more keywords

A Novel PNGase Rc for Improved Protein N-Deglycosylation in Bioanalytics and Hydrogen-Deuterium Exchange Coupled With Mass Spectrometry Epitope Mapping under Challenging Conditions.

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recommendations! Published in 2022 at "Analytical chemistry"

DOI: 10.1021/acs.analchem.2c01748

Abstract: N-linked glycosylation is a ubiquitous posttranslational modification of proteins. While it plays an important role in the biological function of proteins, it often poses a major challenge for their analytical characterization. Currently available peptide N-glycanases… read more here.

Keywords: mass spectrometry; hydrogen deuterium; pngase; deuterium exchange ... See more keywords
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Discovery of Highly Active Recombinant PNGase H+ Variants Through the Rational Exploration of Unstudied Acidobacterial Genomes

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recommendations! Published in 2020 at "Frontiers in Bioengineering and Biotechnology"

DOI: 10.3389/fbioe.2020.00741

Abstract: Peptide-N4-(N-acetyl-β-glucosaminyl) asparagine amidases (PNGases, N-glycanases, EC 3.5.1.52) are indispensable tools in releasing N-glycans from glycoproteins. So far, only a limited number of PNGase candidates are available for the structural analysis of glycoproteins and their glycan… read more here.

Keywords: active recombinant; highly active; pngase; pngase variants ... See more keywords