The Number of Point Mutations In iPS Cells and ntES Cells Depends on the Method and Somatic Cell Type Employed for Their Generation.
Sign Up to like & getrecommendations! Published in 2017 at "Stem Cells"
DOI: 10.1002/stem.2601
Abstract: Induced pluripotent stem cells hold great promise for regenerative medicine but point mutations have been identified in these cells and have raised serious concerns about their safe use. We generated nucleartransfer ES cells (ntESCs) from… read more here.
Keywords: medicine; point mutations; ips cells; number ... See more keywords
The Quality of Genetic Code Models in Terms of Their Robustness Against Point Mutations
Sign Up to like & getrecommendations! Published in 2019 at "Bulletin of Mathematical Biology"
DOI: 10.1007/s11538-019-00603-2
Abstract: In this paper, we investigate the quality of selected models of theoretical genetic codes in terms of their robustness against point mutations. To deal with this problem, we used a graph representation including all possible… read more here.
Keywords: quality; code; point mutations; code models ... See more keywords
Detection of low-abundance point mutations by competitive strand assisted endonuclease IV signal amplification system
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DOI: 10.1007/s11596-017-1808-7
Abstract: SummaryGenetic mutations are important molecular biomarkers for cancer diagnosis and surveillance. Therefore, the development of methods for mutation detection characterized with straightforward, highly specific and sensitive to low-level mutations within various sequence contexts is extremely… read more here.
Keywords: detection; mutations competitive; point mutations; detection low ... See more keywords
Temperature sensitive point mutations in fission yeast tropomyosin have long range effects on the stability and function of the actin-tropomyosin copolymer
Sign Up to like & getrecommendations! Published in 2018 at "Biochemical and Biophysical Research Communications"
DOI: 10.1016/j.bbrc.2017.10.109
Abstract: The actin cytoskeleton is modulated by regulatory actin-binding proteins which fine-tune the dynamic properties of the actin polymer to regulate function. One such actin-binding protein is tropomyosin (Tpm), a highly-conserved alpha-helical dimer which stabilises actin… read more here.
Keywords: point mutations; tropomyosin; temperature sensitive; function ... See more keywords
Improving the Accuracy of Protein Thermostability Predictions for Single Point Mutations.
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DOI: 10.1016/j.bpj.2020.05.020
Abstract: Accurately predicting the protein thermostability changes upon single point mutations in silico is a challenge that has implications for understanding diseases as well as industrial applications of protein engineering. Free energy perturbation (FEP) has been applied… read more here.
Keywords: stability; point mutations; accuracy; protein thermostability ... See more keywords
NTRK point mutations and their functional consequences.
Sign Up to like & getrecommendations! Published in 2021 at "Cancer genetics"
DOI: 10.1016/j.cancergen.2021.12.002
Abstract: The neurotrophic receptor tyrosine kinase (NTRK) family of genes, including NTRK1, NTRK2, and NTRK3, encodes membrane-bound receptors that normally regulate cell survival and differentiation upon binding of growth factors. Not surprisingly, mutations in these genes… read more here.
Keywords: mutations functional; point; point mutations; ntrk point ... See more keywords
Rational design of low immunogenic anti CD25 recombinant immunotoxin for T cell malignancies by elimination of T cell epitopes in PE38.
Sign Up to like & getrecommendations! Published in 2017 at "Cellular immunology"
DOI: 10.1016/j.cellimm.2017.01.003
Abstract: LMB-2, is a potent recombinant immunotoxin (RIT) that is composed of scFv antibody that targets CD25 (Tac) and a toxin fragment (PE38). It is used to treat T cell leukemias and lymphomas. To make LMB-2… read more here.
Keywords: recombinant immunotoxin; lmb 142; cell epitopes; point mutations ... See more keywords
Generation of Targeted Point Mutations in Rice by a Modified CRISPR/Cas9 System.
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DOI: 10.1016/j.molp.2016.12.001
Abstract: CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated Cas9 endonuclease)-mediated genome editing has revolutionized biological research and crop improvement because of its specificity, simplicity, and versatility (reviewed in Komor et al., 2016a). Editing a gene by… read more here.
Keywords: targeted point; point mutations; crispr cas9; generation targeted ... See more keywords
Improving the mutagenesis efficiency of the Kunkel method by codon optimization and annealing temperature adjustment.
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DOI: 10.1016/j.nbt.2019.11.004
Abstract: The Kunkel method is a widely used site-directed mutagenesis strategy that introduces point mutations by annealing mutation-containing oligonucleotides to single-stranded uracil-containing DNA (dU-ssDNA) templates. The method is fast and inexpensive and has been routinely employed… read more here.
Keywords: mutagenesis; point mutations; kunkel method; improving mutagenesis ... See more keywords
Pluripotent cells display enhanced resistance to mutagenesis.
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DOI: 10.1016/j.scr.2016.12.029
Abstract: Pluripotent cells have been reported to exhibit lower frequencies of point mutations and higher levels of DNA repair than differentiated cells. This predicts that pluripotent cells are less susceptible to mutagenic exposures than differentiated cells.… read more here.
Keywords: pluripotent cells; point mutations; pluripotent; cells display ... See more keywords
Generation of R272Q, S156A and K572R RHOT1/Miro1 point mutations in iPSCs from a healthy individual using FACS-assisted CRISPR/Cas9 genome editing.
Sign Up to like & getrecommendations! Published in 2021 at "Stem cell research"
DOI: 10.1016/j.scr.2021.102469
Abstract: The GTPase Miro1 is tail anchored into the mitochondrial outer membrane and tethers mitochondria to molecular motors which is crucial for mitochondrial transport. Miro1 contains two EF hand, ion binding domains important for calcium sequestration.… read more here.
Keywords: k572r; point mutations; r272q s156a; generation r272q ... See more keywords