Detection of the Polar Body After Fertilization.
Sign Up to like & getrecommendations! Published in 2021 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0970-5_13
Abstract: The polar body, with haploid DNA, is a small cell produced during the meiosis of an oocyte. Here, we describe the detailed procedures for the detection of the second polar body in zebrafish (Danio rerio)… read more here.
Keywords: detection polar; body fertilization; polar body; biology ... See more keywords
POLAR BODY ANALYSIS FOR PREIMPLANTATION GENETIC TESTING
Sign Up to like & getrecommendations! Published in 2019 at "Reproductive BioMedicine Online"
DOI: 10.1016/j.rbmo.2019.04.028
Abstract: The use of polar bodies for preimplantation genetic testing for both single gene defects (PGT-M) and aneuploidy (PGT-A) was pioneered by Verlinsky and colleagues beginning in the late 1980s. For many years, it was applied… read more here.
Keywords: body; genetic testing; preimplantation genetic; polar body ... See more keywords
Functional Human Oocytes Generated by Transfer of Polar Body Genomes.
Sign Up to like & getrecommendations! Published in 2017 at "Cell stem cell"
DOI: 10.1016/j.stem.2016.10.001
Abstract: Oocyte defects lie at the heart of some forms of infertility and could potentially be addressed therapeutically by alternative routes for oocyte formation. Here, we describe the generation of functional human oocytes following nuclear transfer… read more here.
Keywords: oocytes generated; functional human; human oocytes; polar body ... See more keywords
178 Evaluation of polar body extrusion following exposure of cattle oocytes to different concentrations of ethylene glycol cryoprotectant
Sign Up to like & getrecommendations! Published in 2020 at "Reproduction, Fertility and Development"
DOI: 10.1071/rdv32n2ab178
Abstract: Cryoprotectant (CPA) toxicity has been recognised as a critical barrier to further advancement of cattle oocytes. Furthermore, CPA at high concentration might be toxic to oocytes, leading to osmotic shock and cell death (Arav et… read more here.
Keywords: body extrusion; bcb; polar body; toxicity ... See more keywords
129 Polar body extrusion following exposure of pig oocytes to different invitro maturation media
Sign Up to like & getrecommendations! Published in 2021 at "Reproduction, Fertility and Development"
DOI: 10.1071/rdv33n2ab129
Abstract: The invitro maturation (IVM) rate of pig oocytes is a critical step for subsequent invitro embryo production. The objective of the present study was to compare different IVM media (NCSU 23, NCSU 37, TCM-199, pFF,… read more here.
Keywords: body extrusion; pig oocytes; body; maturation ... See more keywords
A localized calcium transient and polar body abscission
Sign Up to like & getrecommendations! Published in 2022 at "Cell Cycle"
DOI: 10.1080/15384101.2022.2092815
Abstract: ABSTRACT Polar body emission is a special form of cytokinesis in oocyte meiosis that ensures the correct number of chromosomes in reproduction-competent eggs. The molecular mechanism of the last step, polar body abscission, is poorly… read more here.
Keywords: abscission; calcium; calcium transient; polar body ... See more keywords
The Effect of Epigenetic Changes on the Extrusion of the First Polar Body in Pig Oocytes During In Vitro Maturation.
Sign Up to like & getrecommendations! Published in 2019 at "Cellular reprogramming"
DOI: 10.1089/cell.2018.0071
Abstract: The present study was designed to investigate the comprehensive function of maternal factors of primordial germ cell 7 (PGC7) and POU5F1-POU class 5 homeobox 1 (OCT4), as well as the epigenetic modification roles on the… read more here.
Keywords: pig; extrusion first; first polar; polar body ... See more keywords
Correlation of the position and status of the polar body from the fertilized oocyte to the euploid status of blastocysts
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Genetics"
DOI: 10.3389/fgene.2022.1006870
Abstract: Polar bodies are tiny cells that are extruded during oocyte meiosis and are generally considered not essential for embryonic development. Therefore, polar bodies have been widely used as important materials for the preimplantation genetic diagnosis… read more here.
Keywords: correlation position; polar body; status; position status ... See more keywords