Direct Detection of Products from S-Adenosylmethionine-Dependent Enzymes Using a Competitive Fluorescence Polarization Assay.
Sign Up to like & getrecommendations! Published in 2018 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.7b03556
Abstract: S-Adenosylmethionine (AdoMet)-dependent methyltransferases (MTases) are an essential superfamily of enzymes that catalyze the transfer of a methyl group to several biomolecules. Alterations in the methylation of cellular components crucially impact vital biological processes, making MTases… read more here.
Keywords: dependent enzymes; competitive fluorescence; fluorescence polarization; polarization assay ... See more keywords
A Fluorescence Polarization Assay for Macrodomains Facilitates the Identification of Potent Inhibitors of the SARS-CoV-2 Macrodomain
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DOI: 10.1021/acschembio.3c00092
Abstract: Viral macrodomains, which can bind to and/or hydrolyze adenine diphosphate ribose (ADP-ribose or ADPr) from proteins, have been suggested to counteract host immune response and be viable targets for the development of antiviral drugs. Therefore,… read more here.
Keywords: polarization assay; cov macro1; sars cov; fluorescence polarization ... See more keywords
Assessment of fluorescence polarization assay: a candid diagnostic tool in Brucella abortus strain 19 vaccinated areas
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DOI: 10.1111/1348-0421.12654
Abstract: Brucellosis caused by the bacteria of the genus Brucella is an important zoonosis and constitutes a serious public health hazard. Brucellosis is diagnosed mainly by the Rose Bengal plate test and indirect ELISA, both of… read more here.
Keywords: brucella; fluorescence polarization; polarization assay; assessment fluorescence ... See more keywords
Evaluation of the Fluorescence Polarization Assay for the Diagnosis of Brucellosis in Goat Milk
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DOI: 10.3390/vetsci9060303
Abstract: The milk ring test is a detection assay for antibodies against Brucella in bovine milk. It has good sensitivity but tends to give false positive results. In this study, we standardized the application of the… read more here.
Keywords: goat; polarization assay; goat milk; test ... See more keywords