Vesicle trafficking in Arabidopsis pollen tubes.
Sign Up to like & getrecommendations! Published in 2022 at "FEBS letters"
DOI: 10.1002/1873-3468.14343
Abstract: The delivery of sperm cells via tip-growing pollen tubes is an innovation of seed plants and shows the importance of pollen tubes for reproduction as well as their specific growth kinetics. Fast-growing pollen tubes demand… read more here.
Keywords: pollen tubes; pollen; trafficking arabidopsis; arabidopsis pollen ... See more keywords
Analyzing the Impact of Protein Overexpression on Ca2+ Dynamics and Development in Tobacco Pollen Tubes.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0672-8_16
Abstract: Overexpression of RFP-tagged proteins in growing tobacco pollen tubes together with the genetically encoded Ca2+ sensor YC3.6 allows to analyze localization and dynamics of the protein of interest, as well as the impact of its… read more here.
Keywords: overexpression; tobacco pollen; ca2; pollen tubes ... See more keywords
Flow Chamber Assay to Image the Response of FRET-Based Nanosensors in Pollen Tubes to Changes in Medium Composition.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0672-8_19
Abstract: Pollen tubes growing in the transmitting tract are presented with an extracellular matrix rich in a variety of substances. The expression of a multitude of genes for transport proteins in the pollen tube indicates that… read more here.
Keywords: based nanosensors; pollen tubes; pollen; chamber assay ... See more keywords
Analysis of Actin-Based Intracellular Trafficking in Pollen Tubes.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7262-3_11
Abstract: Underlying rapid and directional pollen tube growth is the active intracellular trafficking system that carries materials necessary for cell wall synthesis and membrane expansion to the expanding point of the pollen tube. The actin cytoskeleton… read more here.
Keywords: intracellular trafficking; actin based; analysis actin; pollen tubes ... See more keywords
CsHT11 encodes a pollen-specific hexose transporter and is induced under high level sucrose in pollen tubes of cucumber (Cucumis sativus)
Sign Up to like & getrecommendations! Published in 2020 at "Plant Growth Regulation"
DOI: 10.1007/s10725-020-00573-9
Abstract: Pollen tubes require a high amount of sugars to sustain high growth rate. Sucrose, the main transport form of sugars, can serve as energy supply and as signaling molecules for pollen tube growth. We report… read more here.
Keywords: pollen tubes; hexose transporter; csht11; pollen specific ... See more keywords
Arabidopsis AIP1-1 regulates the organization of apical actin filaments by promoting their turnover in pollen tubes
Sign Up to like & getrecommendations! Published in 2019 at "Science China Life Sciences"
DOI: 10.1007/s11427-019-9532-0
Abstract: Apical actin filaments are highly dynamic structures that are crucial for rapid pollen tube growth, but the mechanisms regulating their dynamics and spatial organization remain incompletely understood. We here identify that AtAIP1-1 is important for… read more here.
Keywords: actin; apical actin; pollen tubes; actin filaments ... See more keywords
AGC1.5 Kinase Phosphorylates RopGEFs to Control Pollen Tube Growth.
Sign Up to like & getrecommendations! Published in 2018 at "Molecular plant"
DOI: 10.1016/j.molp.2018.07.004
Abstract: Double fertilization in angiosperms requires the targeted delivery of immotile sperm to the eggs through pollen tubes. The polarity of tip-growing pollen tubes is maintained through dynamic association of active Rho GTPases of plants (ROP-GTP)… read more here.
Keywords: pollen; tube growth; pollen tubes; growth ... See more keywords
Quantitative assessment of chemotropism in pollen tubes using microslit channel filters
Sign Up to like & getrecommendations! Published in 2018 at "Biomicrofluidics"
DOI: 10.1063/1.5023718
Abstract: We present a semi-in vitro chemotropism assay that can be used to evaluate the chemoattractant effect of diffusible plant signaling molecules on growing pollen tubes. We constructed an array of microslit channels in a microfluidic… read more here.
Keywords: microslit channel; quantitative assessment; assessment chemotropism; pollen tubes ... See more keywords
Actin Cytoskeleton in the Control of Vesicle Transport, Cytoplasmic Organization and Pollen Tube Tip Growth.
Sign Up to like & getrecommendations! Published in 2023 at "Plant physiology"
DOI: 10.1093/plphys/kiad203
Abstract: Pollen tubes extend rapidly via tip growth. This process depends on a dynamic actin cytoskeleton, which has been implicated in controlling organelle movements, cytoplasmic streaming, vesicle trafficking, and cytoplasm organization in pollen tubes. In this… read more here.
Keywords: pollen tubes; organization pollen; actin cytoskeleton; pollen ... See more keywords
H2 O2 in tobacco stigma exudate affects pollen proteome and membrane potential in pollen tubes.
Sign Up to like & getrecommendations! Published in 2021 at "Plant biology"
DOI: 10.1111/plb.13255
Abstract: ROS are known to be accumulated in stigmas of different species and can possibly perform different functions important for plant reproduction. Here we tested the assumption that one of their functions is to control membrane… read more here.
Keywords: pollen proteome; pollen tubes; membrane potential; stigma exudate ... See more keywords
RALF peptide signaling controls the polytubey block in Arabidopsis
Sign Up to like & getrecommendations! Published in 2022 at "Science"
DOI: 10.1126/science.abl4683
Abstract: Fertilization of an egg by multiple sperm (polyspermy) leads to lethal genome imbalance and chromosome segregation defects. In Arabidopsis thaliana, the block to polyspermy is facilitated by a mechanism that prevents polytubey (the arrival of… read more here.
Keywords: pollen tube; fertilization; pollen; pollen tubes ... See more keywords