Aberrant Ki‐67 expression through 3′UTR alternative polyadenylation in breast cancers
Sign Up to like & getrecommendations! Published in 2018 at "FEBS Open Bio"
DOI: 10.1002/2211-5463.12364
Abstract: Ki‐67 (MKI67) is a marker of cellular proliferation of cancer. Here, we show that Ki‐67 is post‐transcriptionally regulated through alternative polyadenylation (APA) and microRNAs in breast cancer. We show that shortening of the Ki‐67 3′UTR… read more here.
Keywords: breast; aberrant expression; utr alternative; polyadenylation ... See more keywords
Birth of a poly(A) tail: Mechanisms and control of mRNA polyadenylation.
Sign Up to like & getrecommendations! Published in 2022 at "FEBS open bio"
DOI: 10.1002/2211-5463.13528
Abstract: During their synthesis in the cell nucleus, most eukaryotic mRNAs undergo a two-step 3'-end processing reaction in which the pre-mRNA is cleaved and released from the transcribing RNA polymerase II and a polyadenosine (poly(A)) tail… read more here.
Keywords: poly tail; birth poly; polyadenylation; end processing ... See more keywords
Emerging roles of alternative cleavage and polyadenylation (APA) in human disease.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of cellular physiology"
DOI: 10.1002/jcp.30549
Abstract: In the messenger RNA (mRNA) maturation process, the 3'-end of pre-mRNA is cleaved and a poly(A) sequence is added, this is an important determinant of mRNA stability and its cellular functions. More than 60%-70% of… read more here.
Keywords: cleavage polyadenylation; polyadenylation apa; polyadenylation; alternative cleavage ... See more keywords
Regulation of Isoform Expression by Blocking Polyadenylation Signal Sequences with Morpholinos.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6817-6_12
Abstract: Alternative polyadenylation is increasingly being recognized as an important layer of gene regulation. Antisense-mediated modulation of alternative polyadenylation represents an attractive strategy for the regulation of gene expression as well as potential therapeutic applications. In… read more here.
Keywords: expression blocking; isoform expression; polyadenylation; polyadenylation signal ... See more keywords
Genome-wide alternative polyadenylation dynamics in response to biotic and abiotic stresses in rice.
Sign Up to like & getrecommendations! Published in 2019 at "Ecotoxicology and environmental safety"
DOI: 10.1016/j.ecoenv.2019.109485
Abstract: Alternative polyadenylation (APA) is an important way to regulate gene expression at the post-transcriptional level, and is extensively involved in plant stress responses. However, the systematic roles of APA regulation in response to abiotic and… read more here.
Keywords: stress responsive; rice; response; apa ... See more keywords
Alternative polyadenylation expands the mRNA isoform repertoire of human CD46.
Sign Up to like & getrecommendations! Published in 2017 at "Gene"
DOI: 10.1016/j.gene.2017.05.004
Abstract: Alternative polyadenylation is a prevalent mechanism regulating mammalian gene expression. While tandem 3'-Untranslated-Region (3'UTR) polyadenylation changes expression levels, Intronic PolyAdenylation generates shorter transcripts encoding truncated proteins. Intronic PolyAdenylation regulates 20% of genes and is especially… read more here.
Keywords: intronic polyadenylation; human cd46; polyadenylation; cd46 ... See more keywords
CPSF30-L-mediated recognition of mRNA m6A modification controls alternative polyadenylation of nitrate signaling-related gene transcripts in Arabidopsis.
Sign Up to like & getrecommendations! Published in 2021 at "Molecular plant"
DOI: 10.1016/j.molp.2021.01.013
Abstract: N6-methyladenosine (m6A), a ubiquitous internal modification of eukaryotic mRNAs, plays a vital role in almost every aspect of mRNA metabolism. However, evidence regarding the role of m6A in regulating alternative polyadenylation (APA) is limited. APA… read more here.
Keywords: cpsf30; m6a modification; nitrate signaling; polyadenylation ... See more keywords
CDK12 loss in cancer cells affects DNA damage response genes through premature cleavage and polyadenylation
Sign Up to like & getrecommendations! Published in 2019 at "Nature Communications"
DOI: 10.1038/s41467-019-09703-y
Abstract: Cyclin-dependent kinase 12 (CDK12) modulates transcription elongation by phosphorylating the carboxy-terminal domain of RNA polymerase II and selectively affects the expression of genes involved in the DNA damage response (DDR) and mRNA processing. Yet, the… read more here.
Keywords: dna damage; polyadenylation; damage response; cdk12 ... See more keywords
FLEP-seq: simultaneous detection of RNA polymerase II position, splicing status, polyadenylation site and poly(A) tail length at genome-wide scale by single-molecule nascent RNA sequencing.
Sign Up to like & getrecommendations! Published in 2021 at "Nature protocols"
DOI: 10.1038/s41596-021-00581-7
Abstract: Elongation, splicing and polyadenylation are fundamental steps of transcription, and studying their coordination requires simultaneous monitoring of these dynamic processes on one transcript. We recently developed a full-length nascent RNA sequencing method in the model… read more here.
Keywords: length; rna; nascent rna; flep seq ... See more keywords
The polyadenylation inhibitor cordycepin reduces pain, inflammation and joint pathology in rodent models of osteoarthritis
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DOI: 10.1038/s41598-019-41140-1
Abstract: Clinically, osteoarthritis (OA) pain is significantly associated with synovial inflammation. Identification of the mechanisms driving inflammation could reveal new targets to relieve this prevalent pain state. Herein, a role of polyadenylation in OA synovial samples… read more here.
Keywords: inflammation; pathology; polyadenylation; inhibitor cordycepin ... See more keywords
Sense-oriented AluYRa1 elements provide a lineage-specific transcription environment for polyadenylation
Sign Up to like & getrecommendations! Published in 2021 at "Scientific Reports"
DOI: 10.1038/s41598-021-83360-4
Abstract: Transposable elements cause alternative splicing (AS) in different ways, contributing to transcript diversification. Alternative polyadenylation (APA), one of the AS events, is related to the generation of mRNA isoforms in 70% of human genes. In… read more here.
Keywords: oriented aluyra1; polyadenylation; sense oriented; environment polyadenylation ... See more keywords