Site directed mutagenesis as a precision tool to enable synthetic biology with engineered modular polyketide synthases
Sign Up to like & getrecommendations! Published in 2020 at "Synthetic and Systems Biotechnology"
DOI: 10.1016/j.synbio.2020.04.001
Abstract: Modular polyketide synthases (PKSs) are a multidomain megasynthase class of biosynthetic enzymes that have great promise for the development of new compounds, from new pharmaceuticals to high value commodity and specialty chemicals. Their colinear biosynthetic… read more here.
Keywords: protein; synthetic biology; modular polyketide; biology ... See more keywords
The Ketosynthase Domain Constrains the Design of Polyketide Synthases.
Sign Up to like & getrecommendations! Published in 2020 at "ACS chemical biology"
DOI: 10.1021/acschembio.0c00405
Abstract: Modular polyketide synthases (PKSs) produce complex, bioactive secondary metabolites in assembly line-like multistep reactions. Longstanding efforts to produce novel, biologically active compounds by recombining intact modules to new modular PKSs have mostly resulted in poorly… read more here.
Keywords: modular pkss; domain; polyketide synthases; pkss ... See more keywords
Biosynthesis-Guided Discovery and Engineering of α-Pyrone Natural Products from Type I Polyketide Synthases
Sign Up to like & getrecommendations! Published in 2023 at "ACS Chemical Biology"
DOI: 10.1021/acschembio.3c00081
Abstract: Natural products containing the α-pyrone moiety are produced by polyketide synthases (PKSs) in bacteria, fungi, and plants. The conserved biosynthetic logic for the production of the α-pyrone moiety involves the cyclization of a triketide intermediate… read more here.
Keywords: biosynthesis guided; pyrone; natural products; natural product ... See more keywords
Chemoinformatic-guided engineering of polyketide synthases.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.0c02549
Abstract: Polyketide synthase (PKS) engineering is an attractive method to generate new molecules such as commodity, fine and spe-cialty chemicals. A significant challenge in PKS design is engineering a partially reductive module to produce a saturated… read more here.
Keywords: engineering; reductive loops; guided engineering; engineering polyketide ... See more keywords
Intrinsic and Extrinsic Programming of Product Chain Length and Release Mode in Fungal Collaborating Iterative Polyketide Synthases.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.0c07050
Abstract: Combinatorial biosynthesis with fungal polyketide synthases (PKSs) promises to produce unprecedented bioactive "unnatural" natural products (uNPs) for drug discovery. Genome mining of the dothideomycete Rhytidhysteron rufulum uncovered a collaborating highly reducing PKS (hrPKS) - nonreducing… read more here.
Keywords: polyketide synthases; product; chain length; pks ... See more keywords
Mechanism of intersubunit ketosynthase-dehydratase interaction in polyketide synthases.
Sign Up to like & getrecommendations! Published in 2018 at "Nature chemical biology"
DOI: 10.1038/nchembio.2549
Abstract: Modular polyketide synthases (PKSs) produce numerous structurally complex natural products that have diverse applications in medicine and agriculture. PKSs typically consist of several multienzyme subunits that utilize structurally defined docking domains (DDs) at their N… read more here.
Keywords: polyketide synthases; ketosynthase dehydratase; mechanism; interaction ... See more keywords
GRINS: Genetic elements that recode assembly-line polyketide synthases and accelerate their diversification
Sign Up to like & getrecommendations! Published in 2021 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2100751118
Abstract: Significance Assembly-line polyketide synthases (PKSs) make many medicinally significant natural products. A better understanding of evolutionary mechanisms underlying polyketide diversification could open new avenues for PKS engineering and drug discovery. In the course of interrogating… read more here.
Keywords: line; diversification; line polyketide; polyketide synthases ... See more keywords
Biosynthesis of polyketides by two type III polyketide synthases from Aloe barbadensis
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Asian Natural Products Research"
DOI: 10.1080/10286020.2019.1674287
Abstract: Abstract Various bioactive polyketides have been found in Aloe barbadensis. However, the polyketide synthases (PKSs), which participate in biosynthesis of polyketides in A. barbadensis remain unknown. In this study, two type III PKSs (AbPKS1 and… read more here.
Keywords: biosynthesis polyketides; two type; barbadensis; polyketide synthases ... See more keywords
Evolutionary Histories of Type III Polyketide Synthases in Fungi
Sign Up to like & getrecommendations! Published in 2019 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2019.03018
Abstract: Type III polyketide synthases (PKSs) produce secondary metabolites with diverse biological activities, including antimicrobials. While they have been extensively studied in plants and bacteria, only a handful of type III PKSs from fungi has been… read more here.
Keywords: iii; iii polyketide; polyketide synthases; gene ... See more keywords