A Single Amino Acid Substitution in Therminator DNA Polymerase Increases Incorporation Efficiency of Deoxyxylonucleotides
Sign Up to like & getrecommendations! Published in 2018 at "ChemBioChem"
DOI: 10.1002/cbic.201800411
Abstract: Deoxyxylonucleic acid (dxNA) is a synthetic polymer that might have potential for heredity and evolution. Because of dxNA's unusual backbone geometry, sequence information stored in it is presumed to be inaccessible to natural nucleic acids… read more here.
Keywords: dna polymerase; incorporation; polymerase; therminator ... See more keywords
Structure‐based pharmacophore modeling, virtual screening approaches to identifying the potent hepatitis C viral protease and polymerase novel inhibitors
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Cellular Biochemistry"
DOI: 10.1002/jcb.30298
Abstract: Hepatitis C is an infectious disease that leads to acute and chronic liver illnesses. Currently, there are no effective vaccines against this deadly virus. Direct acting antiviral (DAA) drugs are given in the combination with… read more here.
Keywords: protease polymerase; polymerase; protease; virtual screening ... See more keywords
In Vitro Assays for RNA Binding and Protein Priming of Hepatitis B Virus Polymerase.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6700-1_13
Abstract: The hepatitis B virus (HBV) polymerase synthesizes the viral DNA genome from the pre-genomic RNA (pgRNA) template through reverse transcription. Initiation of viral DNA synthesis is accomplished via a novel protein priming mechanism, so named… read more here.
Keywords: protein; dna; rna binding; protein priming ... See more keywords
Analysis of Replicative Polymerase Usage by Ribonucleotide Incorporation.
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DOI: 10.1007/978-1-4939-7306-4_18
Abstract: Mapping the usage of replicative DNA polymerases has previously proved to be technically challenging. By exploiting mutant polymerases that incorporate ribonucleotides into the DNA with a significantly higher proficiency than their wild-type counterparts, we and… read more here.
Keywords: replicative polymerase; polymerase usage; incorporation; polymerase ... See more keywords
Molecular insights into RNA-binding properties of Escherichia coli–expressed RNA-dependent RNA polymerase of Antheraea mylitta cytoplasmic polyhedrosis virus
Sign Up to like & getrecommendations! Published in 2017 at "Archives of Virology"
DOI: 10.1007/s00705-017-3412-3
Abstract: Antheraea mylitta cytoplasmic polyhedrosis virus (AmCPV) is responsible for morbidity of the Indian non-mulberry silkworm, A. mylitta. AmCPV belongs to the family Reoviridae and has 11 double-stranded (ds) RNA genome segments (S1-S11). Segment 2 (S2)… read more here.
Keywords: strand rna; rna; rna binding; polymerase ... See more keywords
Computational analysis of the effect of polymerase acidic (PA) gene mutation F35L in the 2009 pandemic influenza A (H1N1) virus on binding aspects of mononucleotides in the endonuclease domain
Sign Up to like & getrecommendations! Published in 2017 at "Archives of Virology"
DOI: 10.1007/s00705-017-3681-x
Abstract: An F35L mutation in the N-terminal domain of the polymerase acidic protein (PA-Nter), which contains the active site of the endonuclease, has been reported to result in higher polymerase activity in mouse-adapted strains of the… read more here.
Keywords: polymerase acidic; influenza h1n1; pandemic influenza; polymerase ... See more keywords
Interaction of the intrinsically disordered C-terminal domain of the sesbania mosaic virus RNA-dependent RNA polymerase with the viral protein P10 in vitro: modulation of the oligomeric state and polymerase activity
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DOI: 10.1007/s00705-019-04163-7
Abstract: The RNA-dependent RNA polymerase (RdRp) of sesbania mosaic virus (SeMV) was previously shown to interact with the viral protein P10, which led to enhanced polymerase activity. In the present investigation, the equilibrium dissociation constant for… read more here.
Keywords: rdrp; polymerase activity; polymerase; p10 ... See more keywords
Identification of a novel small-molecule compound targeting the influenza A virus polymerase PB1-PB2 interface
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DOI: 10.1016/j.antiviral.2016.11.005
Abstract: &NA; The PB1 C‐terminal domain and PB2 N‐terminal domain interaction of the influenza A polymerase, which modulates the assembly of PB1 and PB2 subunits, may serve as a valuable target for the development of novel… read more here.
Keywords: small molecule; pb1 pb2; polymerase; novel small ... See more keywords
Structural characterization of the interaction between the C-terminal domain of the influenza polymerase PA subunit and an optimized small peptide inhibitor.
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DOI: 10.1016/j.antiviral.2020.104971
Abstract: Influenza viruses can cause severe respiratory infections in humans, leading to nearly half a million deaths worldwide each year. Improved antiviral drugs are needed to address the threat of development of novel pandemic strains. Current… read more here.
Keywords: influenza polymerase; polymerase; terminal domain; polymerase subunit ... See more keywords
Influenza polymerase inhibitor resistance: Assessment of the current state of the art - A report of the isirv Antiviral group.
Sign Up to like & getrecommendations! Published in 2021 at "Antiviral research"
DOI: 10.1016/j.antiviral.2021.105158
Abstract: It is more than 20 years since the neuraminidase inhibitors, oseltamivir and zanamivir were approved for the treatment and prevention of influenza. Guidelines for global surveillance and methods for evaluating resistance were established initially by… read more here.
Keywords: inhibitor; polymerase; resistance; isirv antiviral ... See more keywords
Template requirements of Zika RNA polymerase during in vitro RNA synthesis from the 3'-end of virus minus-strand RNA.
Sign Up to like & getrecommendations! Published in 2021 at "Biochimie"
DOI: 10.1016/j.biochi.2021.11.003
Abstract: As ZIKV continues to spread, many "unknowns" remain and research is needed to advance the understanding of this important pathogen. Viral RNA dependent-RNA polymerases (RdRp) are validated targets for inhibitors of the replication of several… read more here.
Keywords: minus strand; rna synthesis; strand rna; rna ... See more keywords