Target-Activated DNA Polymerase Activity for Sensitive RNase H Activity Assay.
Sign Up to like & getrecommendations! Published in 2019 at "Biotechnology journal"
DOI: 10.1002/biot.201800645
Abstract: Herein, the ribonuclease H (RNase H) activity assay based on the target-activated DNA polymerase activity is described. In this method, a detection probe composed of two functional sequences, a binding site for DNA polymerase and… read more here.
Keywords: rnase activity; rnase; dna polymerase; activity ... See more keywords
Interaction of the intrinsically disordered C-terminal domain of the sesbania mosaic virus RNA-dependent RNA polymerase with the viral protein P10 in vitro: modulation of the oligomeric state and polymerase activity
Sign Up to like & getrecommendations! Published in 2019 at "Archives of Virology"
DOI: 10.1007/s00705-019-04163-7
Abstract: The RNA-dependent RNA polymerase (RdRp) of sesbania mosaic virus (SeMV) was previously shown to interact with the viral protein P10, which led to enhanced polymerase activity. In the present investigation, the equilibrium dissociation constant for… read more here.
Keywords: rdrp; polymerase activity; polymerase; p10 ... See more keywords
Label-free monitoring of DNA polymerase activity based on a thrombin-binding aptamer G-quadruplex.
Sign Up to like & getrecommendations! Published in 2017 at "Molecular and cellular probes"
DOI: 10.1016/j.mcp.2016.10.010
Abstract: We have developed a label-free assay for the detection of DNA polymerase activity based on a thrombin-binding aptamer (TBA) G-quadruplex. In the presence of DNA polymerase, the 3'-OH termini of the hairpin substrate are immediately… read more here.
Keywords: based thrombin; activity based; dna polymerase; polymerase ... See more keywords
Noncanonical prokaryotic X family DNA polymerases lack polymerase activity and act as exonucleases
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac461
Abstract: Abstract The X family polymerases (PolXs) are specialized DNA polymerases that are found in all domains of life. While the main representatives of eukaryotic PolXs, which have dedicated functions in DNA repair, were studied in… read more here.
Keywords: polymerase activity; dna polymerases; dna; polxs ... See more keywords
Enhanced polymerase activity permits efficient synthesis by cancer-associated DNA polymerase ϵ variants at low dNTP levels
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac602
Abstract: Abstract Amino acid substitutions in the exonuclease domain of DNA polymerase ϵ (Polϵ) cause ultramutated tumors. Studies in model organisms suggested pathogenic mechanisms distinct from a simple loss of exonuclease. These mechanisms remain unclear for… read more here.
Keywords: cancer associated; polymerase; dna polymerase; polymerase activity ... See more keywords
The Borna Disease Virus 2 (BoDV-2) Nucleoprotein Is a Conspecific Protein That Enhances BoDV-1 RNA-Dependent RNA Polymerase Activity
Sign Up to like & getrecommendations! Published in 2021 at "Journal of Virology"
DOI: 10.1128/jvi.00936-21
Abstract: Borna disease virus 1 (BoDV-1), a prototype virus of the species Mammalian 1 orthobornavirus, is a nonsegmented negative-strand RNA virus that persists in the host nucleus. The nucleoprotein (N) of BoDV-1 encapsidates genomic and antigenomic… read more here.
Keywords: bodv; virus; genetics; polymerase activity ... See more keywords
KPNA6 is a Cofactor of ANP32A/B in Supporting Influenza Virus Polymerase Activity
Sign Up to like & getrecommendations! Published in 2022 at "Microbiology Spectrum"
DOI: 10.1128/spectrum.02073-21
Abstract: Host factor ANP32 plays a fundamental role in supporting the polymerase activity of influenza viruses, but the underlying mechanism is largely unknown. Here, we propose that KPNA6 is involved in the function of ANP32A/B to… read more here.
Keywords: influenza virus; polymerase; polymerase activity;
Investigating Influenza Virus Polymerase Activity in Feline Cells Based on the Influenza Virus Minigenome Replication System Driven by the Feline RNA Polymerase I Promoter
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Immunology"
DOI: 10.3389/fimmu.2022.827681
Abstract: Emerging influenza virus poses a health threat to humans and animals. Domestic cats have recently been identified as a potential source of zoonotic influenza virus. The influenza virus minigenome replication system based on the ribonucleic… read more here.
Keywords: promoter; polymerase; polymerase activity; rna ... See more keywords