Rapid Visual Detection of Benzimidazole Resistance In Botrytis cinerea By Recombinase Polymerase Amplification Combined With A Lateral Flow Dipstick.
Sign Up to like & getrecommendations! Published in 2021 at "Pest management science"
DOI: 10.1002/ps.6697
Abstract: BACKGROUND Benzimidazole resistance in B. cinerea is related to the point mutations in the target β-tubulin gene (TUB2). Three mutations (E198A, E198K, E198V) at codon 198 account for most of the resistant strains. A rapid… read more here.
Keywords: polymerase amplification; benzimidazole resistance; recombinase polymerase; resistance ... See more keywords
Recombinase polymerase amplification-lateral flow (RPA-LF) assay combined with immunomagnetic separation for rapid visual detection of Vibrio parahaemolyticus in raw oysters
Sign Up to like & getrecommendations! Published in 2020 at "Analytical and Bioanalytical Chemistry"
DOI: 10.1007/s00216-020-02532-9
Abstract: This study was the first attempt to optimize a recombinase polymerase amplification (RPA) and lateral flow (LF) assay combined with immunomagnetic separation (IMS) for the detection of Vibrio parahaemolyticus in raw oysters. The newly developed… read more here.
Keywords: detection; polymerase amplification; recombinase polymerase; rpa assay ... See more keywords
Development and evaluation of a rapid recombinase polymerase amplification assay for the detection of human enterovirus 71
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DOI: 10.1007/s00705-018-3859-x
Abstract: Enterovirus 71 (EV71) is one of the most common pathogens of hand, foot, and mouth disease (HFMD). A rapid reverse transcription recombinase polymerase amplification (RT-RPA) assay was established to detect EV71 subgenotype C4 (EV71-C4). The… read more here.
Keywords: recombinase polymerase; detection; enterovirus; polymerase amplification ... See more keywords
Recombinase polymerase amplification applied to plant virus detection and potential implications.
Sign Up to like & getrecommendations! Published in 2018 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2018.01.021
Abstract: Several isothermal techniques for the detection of plant pathogens have been developed with the advent of molecular techniques. Among them, Recombinase Polymerase Amplification (RPA) is becoming an important technique for the rapid, sensitive and cost-effective… read more here.
Keywords: detection; polymerase amplification; plant; recombinase polymerase ... See more keywords
Rapid detection of Enterocytozoon hepatopenaei in shrimp through an isothermal recombinase polymerase amplification assay
Sign Up to like & getrecommendations! Published in 2020 at "Aquaculture"
DOI: 10.1016/j.aquaculture.2020.734987
Abstract: Abstract Enterocytozoon hepatopenaei (EHP) has caused mortality in Pacific white shrimp (Penaeus vannamei) and resulted in large economic losses in shrimp farming. The best way to avoid this parasitic disease is to find out about… read more here.
Keywords: amplification assay; detection; polymerase amplification; enterocytozoon hepatopenaei ... See more keywords
A novel onsite and visual molecular technique to authenticate saffron (Crocus sativus) and its adulterants based on recombinase polymerase amplification
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DOI: 10.1016/j.foodcont.2019.01.011
Abstract: Abstract Saffron (Crocus sativus), one of the most expensive herbs in China, has been subject to various types of adulteration as a consequence of its high price and limited production. The present work describes a… read more here.
Keywords: saffron; saffron crocus; recombinase polymerase; crocus sativus ... See more keywords
Optimization of an isothermal recombinase polymerase amplification method for real-time detection of Potato virus Y O and N types in potato.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of virological methods"
DOI: 10.1016/j.jviromet.2019.02.006
Abstract: Potato virus Y (PVY) is a global challenge for potato production and the leading cause of seed crop downgrading and rejection for certification. Accurate and timely diagnosis is key to effective control of PVY. Here… read more here.
Keywords: detection; polymerase amplification; potato; recombinase polymerase ... See more keywords
Recombinase polymerase amplification assay for rapid detection of porcine circovirus 3.
Sign Up to like & getrecommendations! Published in 2017 at "Molecular and cellular probes"
DOI: 10.1016/j.mcp.2017.09.001
Abstract: The objective of this study was to develop a real-time recombinase polymerase amplification (rt-RPA) assay for the rapid detection of porcine circovirus 3 (PCV3). Specific RPA primers and exo probes were designed for the cap… read more here.
Keywords: detection; polymerase amplification; pcv3; recombinase polymerase ... See more keywords
Recombinase polymerase amplification with lateral flow strip for detecting Babesia microti infections.
Sign Up to like & getrecommendations! Published in 2021 at "Parasitology international"
DOI: 10.1016/j.parint.2021.102351
Abstract: Babesia microti is one of the most important pathogens causing humans and rodents babesiosis-an emerging tick-borne disease that occurs worldwide. At present, the gold standard for the detection of Babesia is the microscopic examination of… read more here.
Keywords: babesia; polymerase amplification; babesia microti; amplification lateral ... See more keywords
A recombinase polymerase amplification–based assay for rapid detection of Chlamydia psittaci
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DOI: 10.1016/j.psj.2020.11.031
Abstract: Chlamydia psittaci is a zoonotic agent of systemic wasting disease in birds and atypical pneumonia in mammalians including humans, constituting a public health risk. A rapid diagnostic assay would be beneficial in screening C. psittaci… read more here.
Keywords: psittaci; rapid detection; polymerase amplification; recombinase polymerase ... See more keywords
Rapid diagnosis of Theileria annulata by recombinase polymerase amplification combined with a lateral flow strip (LF-RPA) in epidemic regions.
Sign Up to like & getrecommendations! Published in 2017 at "Veterinary parasitology"
DOI: 10.1016/j.vetpar.2017.02.019
Abstract: Rapid and accurate diagnosis of Theileria annulata infection contributes to the formulation of strategies to eradicate this parasite. A simple and efficient diagnostic tool, recombinase polymerase amplification (RPA) combined with a lateral flow (LF) strip,… read more here.
Keywords: diagnosis theileria; polymerase amplification; theileria annulata; combined lateral ... See more keywords