An improved digital polymerase chain reaction protocol to capture low‐copy KRAS mutations in plasma cell‐free DNA by resolving ‘subsampling’ issues
Sign Up to like & getrecommendations! Published in 2017 at "Molecular Oncology"
DOI: 10.1002/1878-0261.12110
Abstract: Genetic alterations responsible for the initiation of cancer may serve as immediate biomarkers for early diagnosis. Plasma levels of cell‐free DNA (cfDNA) in patients with cancer are higher than those in healthy individuals; however, the… read more here.
Keywords: resolving subsampling; free dna; cell free; chain reaction ... See more keywords
Leveraging preserved specimens of Nerodia to infer the spatiotemporal dynamics of Ophidiomyces ophidiicola via quantitative polymerase chain reaction
Sign Up to like & getrecommendations! Published in 2023 at "Ecology and Evolution"
DOI: 10.1002/ece3.9998
Abstract: Abstract Ophidiomyces ophidiicola (Oo) is a fungal pathogen and the causative agent of ophidiomycosis that has affected multiple snake taxa across the United States, Europe, and Asia. Ophidiomycosis has often been referred to as an… read more here.
Keywords: polymerase chain; ophidiomyces ophidiicola; quantitative polymerase; chain reaction ... See more keywords
Isometric artifacts from polymerase chain reaction‐massively parallel sequencing analysis of short tandem repeat loci: An emerging issue from a new technology?
Sign Up to like & getrecommendations! Published in 2022 at "Electrophoresis"
DOI: 10.1002/elps.202100143
Abstract: The recent introduction of polymerase chain reaction (PCR)‐massively parallel sequencing (MPS) technologies in forensics has changed the approach to allelic short tandem repeat (STR) typing because sequencing cloned PCR fragments enables alleles with identical molecular… read more here.
Keywords: chain reaction; polymerase chain; parallel sequencing; massively parallel ... See more keywords
One‐step and sequential SARSCOV‐2 polymerase chain reaction tests would not work every time
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Clinical Laboratory Analysis"
DOI: 10.1002/jcla.24226
Abstract: RT‐PCR is widely used as a diagnostic test for the detection of SARS‐CoV‐2. In this study, we aim to describe the clinical utility of serial PCR testing in the final detection of COVID‐19. read more here.
Keywords: sarscov polymerase; polymerase chain; chain reaction; one step ... See more keywords
Viral respiratory infections diagnosed by multiplex polymerase chain reaction in pediatric patients
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Medical Virology"
DOI: 10.1002/jmv.25379
Abstract: Syndromic diagnosis by multiplex nucleic acid amplification tests is the most practical approach to respiratory tract infections since the symptoms are rarely agent‐specific. The aim of this study was to investigate the respiratory viruses in… read more here.
Keywords: chain reaction; multiplex polymerase; respiratory; polymerase chain ... See more keywords
Assessment of the possible inhibitory effect of carrageenan in human papillomavirus DNA testing by polymerase chain reaction amplification
Sign Up to like & getrecommendations! Published in 2021 at "Journal of Medical Virology"
DOI: 10.1002/jmv.26963
Abstract: We assessed carrageenan's potential to inhibit human papillomavirus (HPV) DNA extraction and amplification in vaginal swab samples collected in a trial, assessing the efficacy of a carrageenan‐based gel against HPV infections. Experiment #1 consisted of… read more here.
Keywords: dna; carrageenan; chain reaction; human papillomavirus ... See more keywords
The impact of cerebrospinal fluid viral polymerase chain reaction testing on the management of adults with viral meningitis: A multi‐center retrospective study
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Medical Virology"
DOI: 10.1002/jmv.28198
Abstract: The aim of this study was to evaluate the role of viral polymerase chain reaction (PCR) testing in patients with aseptic meningitis and identify opportunities for improvement in clinical management. All cerebrospinal fluid samples collected… read more here.
Keywords: chain reaction; polymerase chain; meningitis; cerebrospinal fluid ... See more keywords
Rapid polymerase chain reaction assays for Brevibacillus laterosporus detection
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Basic Microbiology"
DOI: 10.1002/jobm.201900188
Abstract: The identification of the ubiquitous spore‐forming bacterium Brevibacillus laterosporus, whose interest in pharma, agriculture, and other industrial sectors is raising, mostly relies on 16S ribosomal RNA gene sequence analysis. However, due to bacterial gene homology,… read more here.
Keywords: polymerase chain; detection; chain reaction; brevibacillus laterosporus ... See more keywords
Long Fragment Polymerase Chain Reaction.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7060-5_3
Abstract: Polymerase chain reaction (PCR) is an oft-used preparatory technique in amplifying specific DNA regions for downstream analysis. The size of an amplicon was initially limited by errors in nucleotide polymerization and template deterioration during thermal… read more here.
Keywords: pcr; polymerase chain; chain reaction;
Chromatin Immunoprecipitation-Polymerase Chain Reaction (ChIP-PCR) Detects Methylation, Acetylation, and Ubiquitylation in S. pombe.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7546-4_3
Abstract: The distribution of modified histones within the fission yeast Schizosaccharomyces pombe genome is ultimately dependent upon the transcriptional activity and in turn influences the ability of the polymerases to bind and progress through the chromatin… read more here.
Keywords: chromatin; reaction chip; immunoprecipitation polymerase; chain reaction ... See more keywords
Clinical Genetic Testing for Fragile X Syndrome by Polymerase Chain Reaction Amplification and Southern Blot Analyses.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9080-1_2
Abstract: Fragile X syndrome (FXS) is characterized by mental retardation and in the vast majority of cases it is caused by expansion of CGG trinucleotide repeats in the 5' untranslated region (or UTR) in the FMR1 gene… read more here.
Keywords: genetic testing; fragile syndrome; clinical genetic; chain reaction ... See more keywords