T7 Polymerase Expression of Guide RNAs in vivo Allows Exportable CRISPR-Cas9 Editing in Multiple Yeast Hosts.
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DOI: 10.1021/acssynbio.7b00461
Abstract: Efficient guide RNA expression often limits CRISPR-Cas9 implementation in new hosts. To address this limitation in fungal systems, we demonstrate the utility of a T7 polymerase system to effectively express sgRNAs. Initially, we developed a… read more here.
Keywords: guide rna; crispr cas9; polymerase expression; guide ... See more keywords