Characterizing methanol metabolism-related promoters for metabolic engineering of Ogataea polymorpha.
Sign Up to like & getrecommendations! Published in 2021 at "Applied microbiology and biotechnology"
DOI: 10.1007/s00253-021-11665-5
Abstract: Promoters play an important role in regulating gene expression, and construction of microbial cell factories requires multiple promoters for balancing the metabolic pathways. However, there are only a limited number of characterized promoters for gene… read more here.
Keywords: methanol metabolism; polymorpha; ogataea polymorpha; gene expression ... See more keywords
Cryopreservation of Marchantia polymorpha spermatozoa
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Plant Research"
DOI: 10.1007/s10265-018-1059-0
Abstract: The liverwort Marchantia polymorpha has become one of the model organisms, since it has less genetic redundancy, sexual and asexual modes of reproduction and a range of genomic and molecular genetic resources. Cryopreservation of fertile… read more here.
Keywords: polymorpha spermatozoa; polymorpha; cryopreservation; marchantia polymorpha ... See more keywords
Electroinduced Extraction of Human Ferritin Heavy Chain Expressed in Hansenula polymorpha
Sign Up to like & getrecommendations! Published in 2017 at "Applied Biochemistry and Biotechnology"
DOI: 10.1007/s12010-017-2627-9
Abstract: AbstractА protocol for the efficient and selective recovery of human ferritin heavy chain (FTH1) expressed intracellularly in Hansenula polymorpha was developed. It was based on electropermeabilisation and an increase in the cell wall porosity by… read more here.
Keywords: ferritin heavy; heavy chain; human ferritin; polymorpha ... See more keywords
The filter feeder Dreissena polymorpha affects nutrient, silicon, and metal(loid) mobilization from freshwater sediments.
Sign Up to like & getrecommendations! Published in 2017 at "Chemosphere"
DOI: 10.1016/j.chemosphere.2017.02.037
Abstract: Organic sediments in aquatic ecosystems are well known sinks for nutrients, silicon, and metal(loid)s. Organic matter-consuming organisms like invertebrate shredders, grazers, and bioturbators significantly affect element fixation or remobilization by changing redox conditions or binding… read more here.
Keywords: freshwater; silicon metal; mobilization; polymorpha ... See more keywords
Recombination machinery engineering for precise genome editing in methylotrophic yeast Ogataea polymorpha
Sign Up to like & getrecommendations! Published in 2021 at "iScience"
DOI: 10.1016/j.isci.2021.102168
Abstract: Summary Methanol biotransformation can expand biorefinery substrate spectrum other than biomass by using methylotrophic microbes. Ogataea (Hansenula) polymorpha, a representative methylotrophic yeast, attracts much attention due to its thermotolerance, but the low homologous recombination (HR)… read more here.
Keywords: recombination machinery; machinery engineering; methylotrophic yeast; recombination ... See more keywords
Efficient genome editing by CRISPR/Cas9 with a tRNA-sgRNA fusion in the methylotrophic yeast Ogataea polymorpha.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of bioscience and bioengineering"
DOI: 10.1016/j.jbiosc.2017.06.001
Abstract: The methylotrophic yeast Ogataea polymorpha (syn. Hansenula polymorpha) is an attractive industrial non-conventional yeast showing high thermo-tolerance (up to 50°C) and xylose assimilation. However, genetic manipulation of O. polymorpha is often laborious and time-consuming because it… read more here.
Keywords: system; crispr cas9; methylotrophic yeast; polymorpha ... See more keywords
Improved G-AgarTrap: A highly efficient transformation method for intact gemmalings of the liverwort Marchantia polymorpha
Sign Up to like & getrecommendations! Published in 2018 at "Scientific Reports"
DOI: 10.1038/s41598-018-28947-0
Abstract: Liverworts are key species for studies of plant evolution, occupying a basal position among the land plants. Marchantia polymorpha has emerged as a highly studied model liverwort, and many relevant techniques, including genetic transformation, have… read more here.
Keywords: marchantia polymorpha; method; transformation; improved agartrap ... See more keywords
MarpoDB: An Open Registry for Marchantia Polymorpha Genetic Parts
Sign Up to like & getrecommendations! Published in 2017 at "Plant and Cell Physiology"
DOI: 10.1093/pcp/pcw201
Abstract: Marchantia polymorpha is an extant relative of the earliest terrestrial plants and has attracted a substantial interest as a model organism for evolutionary and developmental studies. Given its relatively simple genome, compact gene families, simple… read more here.
Keywords: genetic parts; marpodb; synthetic biology; marchantia polymorpha ... See more keywords
Jasmonate-Related MYC Transcription Factors Are Functionally Conserved in Marchantia polymorpha
Sign Up to like & getrecommendations! Published in 2019 at "Plant Cell"
DOI: 10.1105/tpc.18.00974
Abstract: Despite 450 million years of independent evolution, MYC transcription factors that regulate jasmonate responses are functionally conserved between bryophytes and eudicots. The lipid-derived phytohormone jasmonoyl-isoleucine regulates plant immunity, growth and development in vascular plants by… read more here.
Keywords: myc transcription; functionally conserved; jasmonate; transcription factors ... See more keywords
A Comprehensive Toolkit for Quick and Easy Visualization of Marker Proteins, Protein–Protein Interactions and Cell Morphology in Marchantia polymorpha
Sign Up to like & getrecommendations! Published in 2020 at "Frontiers in Plant Science"
DOI: 10.3389/fpls.2020.569194
Abstract: Even though stable genomic transformation of sporelings and thalli of Marchantia polymorpha is straightforward and efficient, numerous problems can arise during critical phases of the process such as efficient spore production, poor selection capacity of… read more here.
Keywords: protein interactions; comprehensive toolkit; marchantia polymorpha; protein protein ... See more keywords
Comprehensive analysis of peptide-coding genes and initial characterization of an LRR-only microprotein in Marchantia polymorpha
Sign Up to like & getrecommendations! Published in 2023 at "Frontiers in Plant Science"
DOI: 10.3389/fpls.2022.1051017
Abstract: In the past two decades, many plant peptides have been found to play crucial roles in various biological events by mediating cell-to-cell communications. However, a large number of small open reading frames (sORFs) or short… read more here.
Keywords: analysis; lrr; mpsgf10b; polymorpha ... See more keywords