The Melolontha melolontha entomopoxvirus fusolin protein is a chitinâactive lytic polysaccharide monooxygenase that displays extreme stability
Sign Up to like & getrecommendations! Published in 2023 at "FEBS Letters"
DOI: 10.1002/1873-3468.14620
Abstract: Spindles are intracellular crystals of the fusolin protein that enhances the oral virulence of insect poxviruses by disruption of the larval chitinous peritrophic matrix. The enigmatic fusolin protein is classified as a lytic polysaccharide monooxygenase… read more here.
Keywords: lytic polysaccharide; fusolin; fusolin protein; extreme stability ... See more keywords
Unfolding of CBP21, a lytic polysaccharide monooxygenase, without dissociation of its copper ion cofactor.
Sign Up to like & getrecommendations! Published in 2019 at "Biopolymers"
DOI: 10.1002/bip.23339
Abstract: Chitin-binding protein 21 (CBP21) from Serratia marcescens is a lytic polysaccharide monooxygenase that contains a copper ion as a cofactor. We aimed to elucidate the unfolding mechanism of CBP21 and the effects of Cu2+ on… read more here.
Keywords: cu2; polysaccharide monooxygenase; copper ion; lytic polysaccharide ... See more keywords
Oxidative cleavage and hydrolytic boosting of cellulose in soybean spent flakes by Trichoderma reesei Cel61A lytic polysaccharide monooxygenase.
Sign Up to like & getrecommendations! Published in 2017 at "Enzyme and microbial technology"
DOI: 10.1016/j.enzmictec.2016.12.007
Abstract: The auxiliary activity family 9 (AA9) copper-dependent lytic polysaccharide monooxygenase (LPMO) from Trichoderma reesei (EG4; TrCel61A) was investigated for its ability to oxidize the complex polysaccharides from soybean. The substrate specificity of the enzyme was… read more here.
Keywords: polysaccharide monooxygenase; lytic polysaccharide; trichoderma reesei; soy spent ... See more keywords
Outer Membrane Vesicle-Mediated Codelivery of the Antifungal HSAF Metabolites and Lytic Polysaccharide Monooxygenase in the Predatory Lysobacter enzymogenes.
Sign Up to like & getrecommendations! Published in 2021 at "ACS chemical biology"
DOI: 10.1021/acschembio.1c00260
Abstract: Lysobacter are new biocontrol agents known for their prolific production of lytic enzymes and bioactive metabolites. L. enzymogenes is a predator of fungi and produces several structurally distinct antimicrobial compounds, such as the antifungal HSAF… read more here.
Keywords: antifungal hsaf; polysaccharide monooxygenase; outer membrane; hsaf ... See more keywords
Mimicking the Cu Active Site of Lytic Polysaccharide Monooxygenase Using Monoanionic Tridentate N-Donor Ligands
Sign Up to like & getrecommendations! Published in 2022 at "ACS Omega"
DOI: 10.1021/acsomega.2c04432
Abstract: In an effort to prepare small molecule mimics of the active site of lytic polysaccharide monooxygenase (LPMO), three monoanionic tridentate N donor ligands comprising a central deprotonated amide group flanked by two neutral donors were… read more here.
Keywords: lytic polysaccharide; site lytic; monoanionic tridentate; active site ... See more keywords
A Lytic Polysaccharide Monooxygenase-like protein functions in fungal copper import and meningitis
Sign Up to like & getrecommendations! Published in 2020 at "Nature chemical biology"
DOI: 10.1038/s41589-019-0437-9
Abstract: Infection by the fungal pathogen Cryptococcus neoformans causes lethal meningitis, primarily in immune-compromised individuals. Colonization of the brain by C. neoformans is dependent on copper (Cu) acquisition from the host, which drives critical virulence mechanisms.… read more here.
Keywords: meningitis; polysaccharide monooxygenase; copper; monooxygenase like ... See more keywords
Production of functionalised chitins assisted by fungal lytic polysaccharide monooxygenase
Sign Up to like & getrecommendations! Published in 2018 at "Green Chemistry"
DOI: 10.1039/c8gc00422f
Abstract: The gene CCT67099 from Fusarium fujikuroi was shown to encode a novel enzyme from the Lytic Polysaccharide Monooxygenase (LPMO) Family AA11. The gene was expressed and a truncated version of the en ... read more here.
Keywords: polysaccharide monooxygenase; lytic polysaccharide; functionalised chitins; production functionalised ... See more keywords
Is density functional theory accurate for lytic polysaccharide monooxygenase enzymes?
Sign Up to like & getrecommendations! Published in 2020 at "Dalton transactions"
DOI: 10.1039/c9dt04486h
Abstract: The lytic polysaccharide monooxygenase (LPMO) enzymes boost polysaccharide depolymerization through oxidative chemistry, which has fueled the hope for more energy-efficient production of biofuel. We have recently proposed a mechanism for the oxidation of the polysaccharide… read more here.
Keywords: density functional; theory; polysaccharide monooxygenase; functional theory ... See more keywords
Mapping the protonation states of the histidine brace in an AA10 lytic polysaccharide monooxygenase using CW-EPR spectroscopy and DFT calculations.
Sign Up to like & getrecommendations! Published in 2022 at "Faraday discussions"
DOI: 10.1039/d1fd00068c
Abstract: The active site of the polysaccharide-degrading lytic polysaccharide monooxygenase (LPMO) enzyme features a single copper ion coordinated by a histidine brace. The primary coordination sphere of the copper contains several ligating atoms which are bonded… read more here.
Keywords: lytic polysaccharide; aa10; histidine brace; spectroscopy ... See more keywords
Characterization of a unique polysaccharide monooxygenase from the plant pathogen Magnaporthe oryzae.
Sign Up to like & getrecommendations! Published in 2023 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2215426120
Abstract: Blast disease in cereal plants is caused by the fungus Magnaporthe oryzae and accounts for a significant loss in food crops. At the outset of infection, expression of a putative polysaccharide monooxygenase (MoPMO9A) is increased.… read more here.
Keywords: domain; polysaccharide monooxygenase; magnaporthe oryzae; mopmo9a ... See more keywords
Lytic polysaccharide monooxygenase increases cellobiohydrolases activity by promoting decrystallization of cellulose surface
Sign Up to like & getrecommendations! Published in 2022 at "Science Advances"
DOI: 10.1126/sciadv.ade5155
Abstract: Efficient depolymerization of crystalline cellulose requires cooperation between multiple cellulolytic enzymes. Through biochemical approaches, molecular dynamics (MD) simulation, and single-molecule observations using high-speed atomic force microscopy (HS-AFM), we quantify and track synergistic activity for cellobiohydrolases… read more here.
Keywords: lytic polysaccharide; surface; activity; polysaccharide monooxygenase ... See more keywords