Enzymatic degradation of algal 1,3-xylan: from synergism of lytic polysaccharide monooxygenases with β-1,3-xylanases to their intelligent immobilization on biomimetic silica nanoparticles
Sign Up to like & getrecommendations! Published in 2020 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-020-10624-w
Abstract: Abstract Lytic polysaccharide monooxygenases (LPMOs) with synergistic effect on polysaccharide hydrolase represent a revolution in biotechnology, which may accelerate the conversion of biomass to the second-generation biofuels. Discovering more hydrolases that have synergism with LPMOs… read more here.
Keywords: immobilization; biomass; synergism; polysaccharide monooxygenases ... See more keywords
A simple enzymatic assay for the quantification of C1-specific cellulose oxidation by lytic polysaccharide monooxygenases
Sign Up to like & getrecommendations! Published in 2019 at "Biotechnology Letters"
DOI: 10.1007/s10529-019-02760-9
Abstract: Objective The development of an enzymatic assay for the specific quantification of the C1-oxidation product, i.e. gluconic acid of cellulose active lytic polysaccharide monooxygenases (LPMOs). Results In combination with a β-glucosidase, the spectrophotometrical assay can… read more here.
Keywords: oxidation; enzymatic assay; assay; polysaccharide monooxygenases ... See more keywords
Lytic polysaccharide monooxygenases as powerful tools in enzymatically assisted preparation of nano-scaled cellulose from lignocellulose: A review.
Sign Up to like & getrecommendations! Published in 2021 at "Bioresource technology"
DOI: 10.1016/j.biortech.2021.126491
Abstract: Nanocellulose, either in the form of fibers or crystals, constitutes a renewable, biobased, biocompatible material with advantageous mechanical properties that can be isolated from lignocellulosic biomass. Enzyme-assisted isolation of nanocellulose is an attractive, environmentally friendly… read more here.
Keywords: scaled cellulose; lytic polysaccharide; preparation nano; polysaccharide monooxygenases ... See more keywords
Enhanced konjac glucomannan hydrolysis by lytic polysaccharide monooxygenases and generating prebiotic oligosaccharides.
Sign Up to like & getrecommendations! Published in 2021 at "Carbohydrate polymers"
DOI: 10.1016/j.carbpol.2020.117241
Abstract: Konjac glucomannan (KGM) hydrolysate is a potentially valuable prebiotic that could improve gastrointestinal health by modulating the growth of probiotic bacteria and by promoting the production of short-chain fatty acids (SCFAs). In this study, we… read more here.
Keywords: enhanced konjac; konjac glucomannan; polysaccharide; polysaccharide monooxygenases ... See more keywords
A novel expression system for lytic polysaccharide monooxygenases.
Sign Up to like & getrecommendations! Published in 2017 at "Carbohydrate research"
DOI: 10.1016/j.carres.2017.02.003
Abstract: Lytic polysaccharide monooxygenases (LPMOs) are key enzymatic players of lignocellulosic biomass degradation processes. As such, they have been introduced in cellulolytic cocktails for more efficient and less expensive lignocellulose saccharification. The recombinant production of LPMOs… read more here.
Keywords: system; expression system; expression; polysaccharide monooxygenases ... See more keywords
Fungal lytic polysaccharide monooxygenases from family AA9: Recent developments and application in lignocelullose breakdown.
Sign Up to like & getrecommendations! Published in 2017 at "International journal of biological macromolecules"
DOI: 10.1016/j.ijbiomac.2017.04.077
Abstract: Fungal lytic polysaccharide monooxygenases (LPMOs) from family AA9 are oxidative enzymes that, in the past few years, have changed the paradigm of cellulose conversion. They are key factor in the lignocellulose breakdown and are widely… read more here.
Keywords: fungal lytic; family aa9; family; polysaccharide monooxygenases ... See more keywords
Polysaccharide degradation by lytic polysaccharide monooxygenases.
Sign Up to like & getrecommendations! Published in 2019 at "Current opinion in structural biology"
DOI: 10.1016/j.sbi.2019.02.015
Abstract: The discovery of oxidative cleavage of glycosidic bonds by enzymes currently known as lytic polysaccharide monooxygenases (LPMOs) has had a major impact on our current understanding of the enzymatic conversion of recalcitrant polysaccharides such as… read more here.
Keywords: polysaccharide degradation; degradation lytic; polysaccharide; biology ... See more keywords
Oligosaccharide binding and thermostability of two related AA9 Lytic Polysaccharide Monooxygenases.
Sign Up to like & getrecommendations! Published in 2020 at "Biochemistry"
DOI: 10.1021/acs.biochem.0c00312
Abstract: Lytic polysaccharide monooxygenases (LPMOs) are copper-dependent enzymes which cleave polysaccharide substrates oxidatively. First discovered because of their action on recalcitrant crystalline substrates (chitin and cellulose) a number of LPMOs are now reported to act on… read more here.
Keywords: thermostability two; oligosaccharide binding; polysaccharide monooxygenases; lytic polysaccharide ... See more keywords
A Random-Sequential Kinetic Mechanism for Polysaccharide Monooxygenases.
Sign Up to like & getrecommendations! Published in 2018 at "Biochemistry"
DOI: 10.1021/acs.biochem.8b00129
Abstract: Polysaccharide monooxygenases (PMOs) are mononuclear copper enzymes that catalyze the hydroxylation of polysaccharides leading to the scission of the glycosidic bond. The mechanism, in which PMOs utilize molecular oxygen to oxidize the polysaccharide substrate, still… read more here.
Keywords: mechanism; random sequential; polysaccharide; sequential kinetic ... See more keywords
Oxidative Cleavage of Glycosidic Bonds by Synthetic Mimics of Lytic Polysaccharide Monooxygenases.
Sign Up to like & getrecommendations! Published in 2022 at "Organic letters"
DOI: 10.1021/acs.orglett.2c01312
Abstract: Lytic polysaccharide monooxygenases (LPMOs) cleave polysaccharides through copper-bound oxyl radicals. We report a synthetic mimic of LPMO that uses micelle-stabilized hydrogen bonds to bind a glycan and two molecularly imprinted hydrophobic pockets to accommodate the… read more here.
Keywords: lytic polysaccharide; glycosidic bonds; oxidative cleavage; cleavage glycosidic ... See more keywords
AA16 Oxidoreductases Boost Cellulose-Active AA9 Lytic Polysaccharide Monooxygenases from Myceliophthora thermophila
Sign Up to like & getrecommendations! Published in 2023 at "ACS Catalysis"
DOI: 10.1021/acscatal.3c00874
Abstract: Copper-dependent lytic polysaccharide monooxygenases (LPMOs) classified in Auxiliary Activity (AA) families are considered indispensable as synergistic partners for cellulolytic enzymes to saccharify recalcitrant lignocellulosic plant biomass. In this study, we characterized two fungal oxidoreductases from… read more here.
Keywords: lytic polysaccharide; myceliophthora thermophila; activity; polysaccharide monooxygenases ... See more keywords