Fluorescent sensors that enable a general method to quantify affinities of receptor proteins for polyubiquitin ligands.
Sign Up to like & getrecommendations! Published in 2019 at "ACS sensors"
DOI: 10.1021/acssensors.9b01240
Abstract: In all eukaryotic cells, modifications of proteins by polymers of ubiquitin (polyUb) are signals used in diverse biological processes. To better understand how polyUb signals are read and promote their different functions, quantitative measurements of… read more here.
Keywords: general method; polyub; fluorescent sensors; sensors enable ... See more keywords
Structure of UBE2K–Ub/E3/polyUb reveals mechanisms of K48-linked Ub chain extension
Sign Up to like & getrecommendations! Published in 2022 at "Nature Chemical Biology"
DOI: 10.1038/s41589-021-00952-x
Abstract: Ubiquitin (Ub) chain types govern distinct biological processes. K48-linked polyUb chains target substrates for proteasomal degradation, but the mechanism of Ub chain synthesis remains elusive due to the transient nature of Ub handover. Here, we… read more here.
Keywords: chain; k48 linked; linked chain; structure ... See more keywords
Polyubiquitination of p62/SQSTM1 is a prerequisite for Fas/CD95 aggregation to promote caspase-dependent apoptosis in cadmium-exposed mouse monocyte RAW264.7 cells
Sign Up to like & getrecommendations! Published in 2019 at "Scientific Reports"
DOI: 10.1038/s41598-019-48684-2
Abstract: Cadmium(Cd) induces cytotoxicity via autophagy-induced apoptosis in non-activated mouse monocytes; however, the molecular mechanism remains unclear. Here, we show that autophagy induces Fas (CD95/APO-1)-mediated apoptosis by promoting accumulation of p62/SQSTM1 in response to Cd. Cd… read more here.
Keywords: fas; apoptosis; polyub; p62 sqstm1 ... See more keywords
Novel polyubiquitin imaging system, PolyUb-FC, reveals that K33-linked polyubiquitin is recruited by SQSTM1/p62
Sign Up to like & getrecommendations! Published in 2018 at "Autophagy"
DOI: 10.1080/15548627.2017.1407889
Abstract: ABSTRACT Ubiquitin chains are formed with 8 structurally and functionally distinct polymers. However, the functions of each polyubiquitin remain poorly understood. We developed a polyubiquitin-mediated fluorescence complementation (PolyUb-FC) assay using Kusabira Green (KG) as a… read more here.
Keywords: sqstm1 p62; polyub; linked polyubiquitin; k33 linked ... See more keywords