Synthesis and purification of linkage-specific polyubiquitin chains of distinct length for structural studies.
Sign Up to like & getrecommendations! Published in 2018 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2018.08.007
Abstract: Polyubiquitylation is one of the most versatile post-translational modifications involved in the regulation of numerous intracellular signaling processes. An assembly procedure that is simple, robust, and efficient to synthesize and purify linkage-specific polyubiquitin chains of… read more here.
Keywords: structural studies; linkage specific; polyubiquitin chains; specific polyubiquitin ... See more keywords
Triubiquitin Probes for Identification of Reader and Eraser Proteins of Branched Polyubiquitin Chains.
Sign Up to like & getrecommendations! Published in 2023 at "ACS chemical biology"
DOI: 10.1021/acschembio.2c00898
Abstract: The important roles played by branched polyubiquitin chains were recently uncovered in proteasomal protein degradation, mitotic regulation, and NF-κB signaling. With the new realization of a wide presence of branched ubiquitin chains in mammalian cells,… read more here.
Keywords: eraser proteins; triubiquitin probes; branched polyubiquitin; polyubiquitin chains ... See more keywords
Shank3 regulates striatal synaptic abundance of Cyld, a deubiquitinase specific for Lys63‐linked polyubiquitin chains
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Neurochemistry"
DOI: 10.1111/jnc.14796
Abstract: The SH3 and multiple ankyrin repeat domains 3 (Shank3) proteins are core organizers of the postsynaptic density in neuronal excitatory synapses, and their defects cause various neurodevelopmental and neuropsychiatric disorders. Mechanistically, Shank3 directly and indirectly… read more here.
Keywords: synaptic abundance; lys63 linked; cyld; polyubiquitin chains ... See more keywords
The deubiquitinase CYLD is a specific checkpoint of the STING antiviral signaling pathway
Sign Up to like & getrecommendations! Published in 2018 at "PLoS Pathogens"
DOI: 10.1371/journal.ppat.1007435
Abstract: Stimulator of interferon genes (STING) is critical for cytosolic DNA-triggered innate immunity. STING is modified by several types of polyubiquitin chains. Here, we report that the deubiquitinase CYLD sustains STING signaling by stabilizing the STING… read more here.
Keywords: cyld; deubiquitinase cyld; signaling pathway; polyubiquitin chains ... See more keywords