Dissecting Molecular Phenotypes Through FACS-Based Pooled CRISPR Screens.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/7651_2021_457
Abstract: Pooled CRISPR screens are emerging as a powerful tool to dissect regulatory networks, by assessing how a protein responds to genetic perturbations in a highly multiplexed manner. A large number of genes are perturbed in… read more here.
Keywords: molecular phenotypes; crispr screens; facs based; dissecting molecular ... See more keywords
Abstract IA08: Pooled CRISPR screens for novel autophagy-related factors identify TMEM41, an ER-resident protein predicted to be a channel
Sign Up to like & getrecommendations! Published in 2020 at "Molecular Cancer Research"
DOI: 10.1158/1557-3125.pi3k-mtor18-ia08
Abstract: The power of forward genetics in yeast is the foundation on which the field of autophagy research firmly stands. Complementary work on autophagy in metazoans has revealed both the deep conservation of this process, as… read more here.
Keywords: crispr screens; crispr; screens novel; novel autophagy ... See more keywords
A benchmark of algorithms for the analysis of pooled CRISPR screens
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DOI: 10.1186/s13059-020-01972-x
Abstract: Genome-wide pooled CRISPR-Cas-mediated knockout, activation, and repression screens are powerful tools for functional genomic investigations. Despite their increasing importance, there is currently little guidance on how to design and analyze CRISPR-pooled screens. Here, we provide… read more here.
Keywords: benchmark algorithms; analysis pooled; pooled crispr; crispr screens ... See more keywords
CRISPR/Cas-based customization of pooled CRISPR libraries
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DOI: 10.1371/journal.pone.0199473
Abstract: Pooled CRISPR libraries are widely used in high-throughput screening to study various biological processes. Various pooled CRISPR libraries have been shared for CRISPR screens and useful tools have been developed to construct researcher’s own libraries,… read more here.
Keywords: pooled crispr; conventional pooled; crispr cas; cas based ... See more keywords