Unraveling docking and initiation of mRNA export through the nuclear pore complex
Sign Up to like & getrecommendations! Published in 2022 at "BioEssays"
DOI: 10.1002/bies.202200027
Abstract: The nuclear export of mRNA through the nuclear pore complex (NPC) is a process required for the healthy functioning of human cells, making it a critical area of research. However, the geometries of mRNA and… read more here.
Keywords: nuclear pore; pore complex; nuclear basket; microscopy ... See more keywords
Nuclear Pore Complex Assembly Using Xenopus Egg Extract.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-2337-4_3
Abstract: Xenopus egg extract is a powerful tool for the in vitro investigation of complex cellular mechanisms. Here we describe how to obtain and employ interphase Xenopus egg extract to study nuclear pore complex assembly and… read more here.
Keywords: xenopus egg; nuclear pore; egg extract; pore complex ... See more keywords
Polymer translocation through a hairy channel mimicking the inner plug of a nuclear pore complex
Sign Up to like & getrecommendations! Published in 2019 at "European Biophysics Journal"
DOI: 10.1007/s00249-019-01356-5
Abstract: A microscopic transport model of a polymer translocating through a nuclear pore complex (NPC) is presented based on self-consistent field theory (SCFT), with the NPC and its nucleoporins mimicked by a hairy channel. Multiple cell… read more here.
Keywords: polymer; pore complex; effect; translocation ... See more keywords
Sol-gel transition in solutions of FG-Nups of the nuclear pore complex
Sign Up to like & getrecommendations! Published in 2018 at "Extreme Mechanics Letters"
DOI: 10.1016/j.eml.2018.04.006
Abstract: The nuclear pore complex (NPC) is a giant molecular gate that regulates the transport of molecules and macromolecules across the nuclear envelop in a selective and directional manner. Using a coarse-grained one-bead-per-amino-acid model and percolation… read more here.
Keywords: solutions nups; pore complex; sol gel; nuclear pore ... See more keywords
Integrative Structure and Functional Anatomy of a Nuclear Pore Complex
Sign Up to like & getrecommendations! Published in 2018 at "Nature"
DOI: 10.1038/nature26003
Abstract: Nuclear pore complexes play central roles as gatekeepers of RNA and protein transport between the cytoplasm and nucleoplasm. However, their large size and dynamic nature have impeded a full structural and functional elucidation. Here we… read more here.
Keywords: pore complex; anatomy; integrative structure; nuclear pore ... See more keywords
Structure of the cytoplasmic ring of the Xenopus laevis nuclear pore complex by cryo-electron microscopy single particle analysis
Sign Up to like & getrecommendations! Published in 2020 at "Cell Research"
DOI: 10.1038/s41422-020-0319-4
Abstract: The nuclear pore complex (NPC) exhibits structural plasticity and has only been characterized at local resolutions of up to 15 Å for the cytoplasmic ring (CR). Here we present a single-particle cryo-electron microscopy (cryo-EM) structure of… read more here.
Keywords: microscopy; pore complex; cytoplasmic ring; nuclear pore ... See more keywords
DNA origami scaffold for studying intrinsically disordered proteins of the nuclear pore complex
Sign Up to like & getrecommendations! Published in 2018 at "Nature Communications"
DOI: 10.1038/s41467-018-03313-w
Abstract: The nuclear pore complex (NPC) is the gatekeeper for nuclear transport in eukaryotic cells. A key component of the NPC is the central shaft lined with intrinsically disordered proteins (IDPs) known as FG-Nups, which control… read more here.
Keywords: dna origami; pore complex; proteins nuclear; nuclear pore ... See more keywords
Mapping paths through the nuclear pore complex
Sign Up to like & getrecommendations! Published in 2022 at "Nature Cell Biology"
DOI: 10.1038/s41556-021-00823-6
Abstract: Nuclear pore complexes (NPCs) facilitate the fast, yet highly selective, nucleocytoplasmic transport of molecules. A recent study describes a multicolour imaging approach to chart the paths for cargo molecules through the human NPC with real-time… read more here.
Keywords: nuclear pore; pore; pore complex; paths nuclear ... See more keywords
In vivo analysis of protein crowding within the nuclear pore complex in interphase and mitosis
Sign Up to like & getrecommendations! Published in 2017 at "Scientific Reports"
DOI: 10.1038/s41598-017-05959-w
Abstract: The central channel of the nuclear pore complex (NPC) is occupied by non-structured polypeptides with a high content of Phe-Gly (FG) motifs. This protein-rich environment functions as an entropic barrier that prevents the passage of… read more here.
Keywords: protein; pore complex; rich domains; protein rich ... See more keywords
The Nuclear Pore Complex 62 Suppression Inhibits Coxsackievirus B Replication and Inflammatory Response.
Sign Up to like & getrecommendations! Published in 2022 at "Viral immunology"
DOI: 10.1089/vim.2021.0194
Abstract: Coxsackievirus B3 (CVB3) is one of the major viruses associated with human viral myocarditis, in members of the Picornaviridae order. Cellular localization depends on the activity of nuclear pore complexes, which are composed of nucleoporins… read more here.
Keywords: replication; cvb3 replication; nuclear pore; pore complex ... See more keywords
Expression of TorsinA in a heterologous yeast system reveals interactions with lumenal domains of LINC and nuclear pore complex components
Sign Up to like & getrecommendations! Published in 2019 at "Molecular Biology of the Cell"
DOI: 10.1091/mbc.e18-09-0585
Abstract: DYT1 dystonia is caused by an in-frame deletion of a glutamic acid codon in the gene encoding the AAA+ ATPase TorsinA (TorA). TorA localizes within the lumen of the nuclear envelope/endoplasmic reticulum and binds to… read more here.
Keywords: nuclear pore; system; tora; tora tora ... See more keywords