High post-thaw survival of ram sperm after partial freeze-drying
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Assisted Reproduction and Genetics"
DOI: 10.1007/s10815-018-1145-1
Abstract: BackgroundRecrystallization damages occur when a frozen sample is held at high subzero temperatures and when the warming process is too slow.MethodsIn this work, ram semen diluted in two different concentrations of sugar solutions (Lyo A… read more here.
Keywords: dried samples; freeze drying; high post; freeze ... See more keywords
Effect of platelet-rich plasma (PRP) on post-thaw quality, kinematics and in vivo fertility of fertile and subfertile buffalo (Bubalus bubalis) spermatozoa.
Sign Up to like & getrecommendations! Published in 2022 at "Veterinary research communications"
DOI: 10.1007/s11259-022-09928-1
Abstract: This study investigated the effect of adding platelet-rich plasma (PRP) in semen extender prior cryopreservation on post-thaw quality, kinematics, and in vivo fertility of fertile and subfertile buffalo spermatozoa. Eleven buffalo bulls were classified based… read more here.
Keywords: quality; fertile subfertile; post thaw; subfertile ... See more keywords
Cryopreserved Cd34 Cells: Measuring Viability Pre-Thaw and Viability over Time Post-Thaw
Sign Up to like & getrecommendations! Published in 2019 at "Biology of Blood and Marrow Transplantation"
DOI: 10.1016/j.bbmt.2018.12.310
Abstract: Common practices associated with the use of cryopreserved hematopoietic progenitor cells (HPC) include measuring the viability of the thawed cells from a sample derived from the thawed product, and thawing cells at the patient's bedside… read more here.
Keywords: viability; cd34 cells; post thaw;
Sperm cryopreservation in the spermcasting Australian flat oyster Ostrea angasi by a programmable freezing method.
Sign Up to like & getrecommendations! Published in 2017 at "Cryobiology"
DOI: 10.1016/j.cryobiol.2017.03.007
Abstract: Cryopreservation offers long-term storage of gametes without constraint from seasonal gamete maturation, provides opportunities to improve the efficiency of breeding and genetic programs, and protects endangered species from extinction due to epidemic diseases and natural… read more here.
Keywords: flat oyster; australian flat; motility; post ... See more keywords
Impact of l-carnitine on lipid content and post thaw survivability of buffalo embryos produced in vitro.
Sign Up to like & getrecommendations! Published in 2018 at "Cryobiology"
DOI: 10.1016/j.cryobiol.2018.04.001
Abstract: The aim of the present study was to see the impact of L-Carnitine (LC) on lipid biosynthesis and metabolism of buffalo embryos, and post thaw blastocyst survivability. In vitro fertilized (IVF) embryos were derived from… read more here.
Keywords: buffalo; carnitine; post; survivability ... See more keywords
Two-step accelerating freezing protocol yields a better motility, membranes and DNA integrities of thawed ram sperm than three-steps freezing protocols.
Sign Up to like & getrecommendations! Published in 2019 at "Cryobiology"
DOI: 10.1016/j.cryobiol.2019.10.007
Abstract: The present study compares a protocol that mimics freezing of ram semen in static nitrogen vapor with two protocols with an initial low cooling rate in the first step, followed by higher cooling rates where… read more here.
Keywords: step; two step; protocol; min ... See more keywords
Effects of Spermine and Spermidine Supplemented Extenders on Post-thaw Spermatological Parameters in Stallion Semen Cryopreservation.
Sign Up to like & getrecommendations! Published in 2021 at "Cryobiology"
DOI: 10.1016/j.cryobiol.2021.03.008
Abstract: In this study, the effects of polyamines, Spermine and Spermidine, on long-term preservation and post-thaw spermatological parameters were evaluated. Moreover, determination of the most suitable polyamine and its dose that can be added to standard… read more here.
Keywords: thaw spermatological; stallion semen; spermine spermidine; spermatological parameters ... See more keywords
The Impact of feeding regimen and cell cycle on post-thaw recovery in a human T-cell model
Sign Up to like & getrecommendations! Published in 2019 at "Cytotherapy"
DOI: 10.1016/j.jcyt.2019.03.384
Abstract: Background & Aim Cell-based therapies require specialized handling to maintain their potency and efficacy during ex vivo transport from the patient to the manufacturing facility and vice versa. Many current approved or in-development therapies employ… read more here.
Keywords: cryopreservation; cell cycle; cell; post thaw ... See more keywords
Low CD45 mean fluorescence intensity predicts poor Post-Thaw CD34 cell viability
Sign Up to like & getrecommendations! Published in 2020 at "Cytotherapy"
DOI: 10.1016/j.jcyt.2020.03.093
Abstract: Background & Aim Background Reduced CD34 cell viability following cryopreservation is associated with suboptimal engraftment in stem cell transplantation. Collection of extra hematopoietic progenitor cells (HPC) can compensate for the potential loss, but subjects patients… read more here.
Keywords: cd34 cell; cd34; post thaw;
Exploring potential FDA-approved components for DMSO-free T-cell cryopreservation solutions
Sign Up to like & getrecommendations! Published in 2020 at "Cytotherapy"
DOI: 10.1016/j.jcyt.2020.03.334
Abstract: Background & Aim The cryopreservation process is an integral part of cellular therapies. However, current DMSO-containing cryomedia are toxic and cause many cyto- and genotoxic such as causing stress at the membrane interface, oxidizing free… read more here.
Keywords: cryomedia; viability; cryopreservation; dmso free ... See more keywords
Post‐thaw Addition of Caffeine and/or Pentoxifylline Affect Differently Motility of Horse and Donkey‐Cryopreserved Spermatozoa
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Equine Veterinary Science"
DOI: 10.1016/j.jevs.2019.01.003
Abstract: ABSTRACT To increase sperm motility, several molecules have been tested in mammals. Methylxanthines have shown effects on sperm motility, capacitation, and on in vitro fertilization processes. The aim of the study was to evaluate if… read more here.
Keywords: pentoxifylline; caffeine pentoxifylline; motility; post thaw ... See more keywords