Co‐translational Installation of Posttranslational Modifications by Non‐canonical Amino Acid Mutagenesis
Sign Up to like & getrecommendations! Published in 2023 at "ChemBioChem"
DOI: 10.1002/cbic.202300039
Abstract: Protein posttranslational modifications (PTMs) play critical roles in regulating cellular activities. Here we provide a survey of genetic code expansion (GCE) methods that were applied in the co‐translational installation and studies of PTMs through noncanonical… read more here.
Keywords: mutagenesis; amino acid; translational installation; posttranslational modifications ... See more keywords
Murburn posttranslational modifications of proteins: Cellular redox processes and murzyme-mediated metabolo-proteomics.
Sign Up to like & getrecommendations! Published in 2023 at "Journal of cellular physiology"
DOI: 10.1002/jcp.30954
Abstract: Murburn concept constitutes the thesis that diffusible reactive species or DRS are obligatorily involved in routine metabolic and physiological activities. Murzymes are defined as biomolecules/proteins that generate/modulate/sustain/utilize DRS. Murburn posttranslational modifications (PTMs) result because murburn/murzyme… read more here.
Keywords: posttranslational modifications; proteins cellular; physiology; murburn posttranslational ... See more keywords
In Silico Analysis of Class III Peroxidases: Hypothetical Structure, Ligand Binding Sites, Posttranslational Modifications, and Interaction with Substrates.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0528-8_24
Abstract: Functional analyses of peroxidases are a major challenge. In silico analysis appears to be a powerful tool to overcome at least some of the problems that arose from (1) the numerous possible functions of peroxidases,… read more here.
Keywords: analysis; class iii; silico analysis; binding sites ... See more keywords
Mass Spectrometry Analysis of Lysine Posttranslational Modifications of Tau Protein from Alzheimer's Disease Brain.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6598-4_10
Abstract: Recent advances in mass spectrometry (MS)-based proteomics have greatly facilitated the robust identification and quantification of posttranslational modifications (PTMs), including those that are present at substoichiometric site occupancies. The abnormal posttranslational modification and accumulation of… read more here.
Keywords: tau; spectrometry; mass spectrometry; alzheimer disease ... See more keywords
The Study of Posttranslational Modifications of Tau Protein by Nuclear Magnetic Resonance Spectroscopy: Phosphorylation of Tau Protein by ERK2 Recombinant Kinase and Rat Brain Extract, and Acetylation by Recombinant Creb-Binding Protein.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6598-4_11
Abstract: Nuclear magnetic resonance (NMR) spectroscopy can be used as an analytical tool to investigate posttranslational modifications of protein. NMR is a valuable tool to map the interaction regions of protein partners. Here, we present protocols… read more here.
Keywords: protein; spectroscopy; posttranslational modifications; phosphorylation ... See more keywords
Detecting Posttranslational Modifications of Hsp90.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7477-1_16
Abstract: The molecular chaperone Heat Shock Protein 90 (Hsp90) is essential in eukaryotes. Hsp90 chaperones proteins that are important determinants of multistep carcinogenesis. The chaperone function of Hsp90 is linked to its ability to bind and… read more here.
Keywords: modifications hsp90; posttranslational modifications; biology; detecting posttranslational ... See more keywords
The Methods Employed in Mass Spectrometric Analysis of Posttranslational Modifications (PTMs) and Protein-Protein Interactions (PPIs).
Sign Up to like & getrecommendations! Published in 2019 at "Advances in experimental medicine and biology"
DOI: 10.1007/978-3-030-15950-4_10
Abstract: Mass Spectrometry (MS) has revolutionized the way we study biomolecules, especially proteins, their interactions and posttranslational modifications (PTM). As such MS has established itself as the leading tool for the analysis of PTMs mainly because… read more here.
Keywords: protein; analysis; mass; biology ... See more keywords
Cataloging Posttranslational Modifications in Plant Histones.
Sign Up to like & getrecommendations! Published in 2021 at "Advances in experimental medicine and biology"
DOI: 10.1007/978-3-030-80352-0_8
Abstract: Eukaryotic DNA exist in the nuclei in the form of a complex with proteins called chromatin. Access to the information encoded in the DNA requires the opening of the chromatin. Modulation of the chromatin structure… read more here.
Keywords: dna; cataloging posttranslational; posttranslational modifications; plant histones ... See more keywords
SnapShot: Functions of Tubulin Posttranslational Modifications
Sign Up to like & getrecommendations! Published in 2018 at "Cell"
DOI: 10.1016/j.cell.2018.05.032
Abstract: Post-translational modification of tubulin offers a mechanism for functional diversification of microtubules and regulation in a variety of physiological contexts. This SnapShot recaps the current state of understanding of tubulin posttranslational modifications and their functions… read more here.
Keywords: posttranslational modifications; functions tubulin; snapshot functions; tubulin posttranslational ... See more keywords
Chemokine isoforms and processing in inflammation and immunity.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of autoimmunity"
DOI: 10.1016/j.jaut.2017.06.009
Abstract: The first dimension of chemokine heterogeneity is reflected by their discovery and purification as natural proteins. Each of those chemokines attracted a specific inflammatory leukocyte type. With the introduction of genomic technologies, a second wave… read more here.
Keywords: chemokine isoforms; inflammation; posttranslational modifications; chemokine heterogeneity ... See more keywords
Nonenzymatic Posttranslational Modifications and Peptide Cleavages Observed in Peptide Epimers.
Sign Up to like & getrecommendations! Published in 2023 at "Journal of the American Society for Mass Spectrometry"
DOI: 10.1021/jasms.3c00092
Abstract: Posttranslational modifications (PTMs) play vital roles in cellular homeostasis and are implicated in various pathological conditions. This work uses two ion mobility spectrometry-mass spectrometry (IMS-MS) modalities, drift-tube IMS (DT-IMS) and trapped IMS (TIMS), to characterize… read more here.
Keywords: source fragmentation; posttranslational modifications; isomerization; source ... See more keywords